Anti-Factor VIII [Clone F827.4]
Anti-Factor VIII [Clone F827.4]
Product No.: 10104
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Clone F827.4 Target Factor VIII Formats AvailableView All Product Type Monoclonal Alternate Names Antihemophilic factor, AHF, Procoagulant component [Cleaved into: Factor VIIIa heavy chain, 200 kDa isoform; Factor VIIIa heavy chain, 92 kDa isoform; Factor VIII B chain; Factor VIIIa light chain] Isotype Mouse IgG2a Applications ELISA , WB |
Data
Detection of Factor VIII light chain (LCh) in wild-type (WT) and recombinant (R372H) Factor VIII after reaction with thrombin over time (Nogami et al. 2005 Blood 105: 4362-4368). - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified human Factor VIII. Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco These antibodies have been qualified for use in ELISA and immunoblotting to detect human Factor VIII. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes an epitope at the N-terminal region of the 83kDa light chain of Factor VIII. These antibodies do not cross-react with von Willebrand factor. Background Factor VIII (FVIII), also known asophilic factor (AHF), is a vital protein for proper blood clotting. The F8 gene is responsible for producing Factor VIII in humans. Defects in this gene lead to hemophilia A, a genetic bleeding disorder that primarily affects males due to its X-linked recessive inheritance pattern. Factor VIII is primarily produced in the liver's sinusoidal cells and in endothelial cells located throughout the body. In its inactive state, Factor VIII circulates in the bloodstream bound to von Willebrand factor (VWF). When a blood vessel is injured, Factor VIII detaches from VWF and becomes activated (FVIIIa). This activated form then collaborates with Factor IX to initiate a cascade of chemical reactions, ultimately leading to the formation of a blood clot and stopping the bleeding. Function Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa. NCBI Gene Bank ID UniProt.org Research Area Coagulation factors References & CitationsTechnical Protocols  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
