Anti-GATA1 (phospho-Ser142) Antibody (43041)
Anti-GATA1 (phospho-Ser142) Antibody (43041)
Product No.: 43041
Data
Immunoblot: Detection of GATA1 (phospho-Ser142) in
extracts of HL60 cells untreated or treated with PMA.
Immunohistochemistry: Detection of GATA1 (phospho-Ser142) in paraffin-embedded human breast carcinoma tissue - -
- -
Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 142 (R-L-S(p)-P-D) derived from human GATA1 and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to GATA1 (phospho-Ser142) Background GATA1 encodes a protein, GATA1, which belongs to the GATA family of transcription factors. This protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Antigen DetailsFunction Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781). {PubMed:22235304, PubMed:24245781}. NCBI Gene Bank ID UniProt.org Research Area Phosphospecific Antibodies References & CitationsTechnical Protocols  |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
