Anti-Glutathione Reductase Antibody (13902)
Anti-Glutathione Reductase Antibody (13902)
Product No.: 13902
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Clone 2A4-1G9 Target Glutathione Reductase Formats AvailableView All Product Type Monoclonal Alternate Names GR, GRase, EC 1.8.1.7 Isotype Mouse IgG1 Applications ELISA , WB |
Data
ELISA: Titration of glutathione reductase MAbs with glutathione reductase at 3ug/ml on the wells.
ELISA Pairs: Both of these antibodies function as capture and detection antibodies at 1ug/ml.
Lane 1: mw standards
Lane 2: Recombinant human glutathione reductase
Lane 3: Lysate of glutathione reductase-transfected 293T cells
A band of ~57kDa is detected. - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Recombinant human glutathione reductase purified from E. coli expressing the human glutathione reductase gene (aa 44-522). Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of PEG purification chromatography. Storage and Handling Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco ELISA: Titration of glutathione reductase MAbs with glutathione reductase at 3ug/ml on the wells. Immunoblotting: use at 0.5-1.0ug/ml. A band of ~57kDa is detected. These are recommended concentrations. End users should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity These antibodies recognize human glutathione reductase. They do not cross-react with yeast glutathione reductase. Background Glutathione reductase, also known as glutathione-disulfide reductase, is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. The importance of the glutathione pathway and enzymes that affect it is gaining an increased level of attention. Although glutathione reductase has been an attractive target for many pharmaceuticals, there have been no successful glutathione reductase related therapeutic compounds created to date. Function Maintains high levels of reduced glutathione in the cytosol. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
