Anti-GPX7 Antibody (13911)

Product No.: 13911


Clone 2704 Target GPX7 Formats AvailableView All Product Type Monoclonal Alternate Names GPx-7, GSHPx-7, EC 1.11.1.9, CL683 Isotype Mouse IgG2a Applications WB,IHC-P,IHC


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Data

Detection of GPX7 using GPX7 antibody [2704] (13911) in HeLa whole cell extract.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX7 antibody [2704] (13911) diluted at 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with GPX7 antibody [2704] (13911) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GPX7 antibody [2704] detects GPX7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GPX7 stained by GPX7 antibody [2704] (13911) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Full length human GPX7 Product Concentration Lot Specific Formulation PBS, pH 7.2. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This product is stable for at least one (1) year if stored at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a 1:500-1:2,000 dilution. A band of ~18kDa is detected. Detection of GPX7 in HeLa cell extract. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity This antibody recognizes human GPX7. Background Glutathione peroxidases (GPXs) are some of the most important enzymes involved in removing reactive oxygen species (ROS). GPX7 (along with GPX5 and GPX8) is a non-selenocysteine glutathione peroxidase. When cells are exposed to oxidative stress, GPX7 acts as an intracellular sensor that detects redox level and transmits ROS signals to redox-sensitive, thiolcontaining proteins to facilitate the regulation of multiple biologic processes such as protein folding and releasing non-targeting short interfering RNAs (siRNAs)-associated stress. Function It protects esophageal epithelia from hydrogen peroxide-induced oxidative stress. It suppresses acidic bile acid-induced reactive oxigen species (ROS) and protects against oxidative DNA damage and double-strand breaks. {PubMed:22157330}. NCBI Gene Bank ID GPX7 UniProt.org Q96SL4 Research Area Enzymes References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Full length human GPX7

Product Concentration

Lot Specific

Formulation

PBS, pH 7.2.

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This product is stable for at least one (1) year if stored at -20°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: use at a 1:500-1:2,000 dilution. A band of ~18kDa is detected. Detection of GPX7 in HeLa cell extract.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes human GPX7.

Background

Glutathione peroxidases (GPXs) are some of the most important enzymes involved in removing reactive oxygen species (ROS). GPX7 (along with GPX5 and GPX8) is a non-selenocysteine glutathione peroxidase. When cells are exposed to oxidative stress, GPX7 acts as an intracellular sensor that detects redox level and transmits ROS signals to redox-sensitive, thiolcontaining proteins to facilitate the regulation of multiple biologic processes such as protein folding and releasing non-targeting short interfering RNAs (siRNAs)-associated stress.

Function

It protects esophageal epithelia from hydrogen peroxide-induced oxidative stress. It suppresses acidic bile acid-induced reactive oxigen species (ROS) and protects against oxidative DNA damage and double-strand breaks. {PubMed:22157330}.

NCBI Gene Bank ID

GPX7

UniProt.org

Q96SL4

Research Area

Enzymes