Anti-Henipavirus (Clone: HENV-103)

Pricing & Details

Product No.LT575
Clone
HENV-103
Protein
Henipavirus
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Isotype
Human IgG1
Applications
FC
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT575-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT575-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Henipavirus
Virus
Expression Host
HEK-293
Immunogen
Sequenced from human samples with prior exposure to equine Hendra virus (HeV) vaccine, targeting distinct antigenic sites.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
FC
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
HENV-103 activity is directed against an area spanning the β1 and β6 propeller blades of receptor binding protein (RBP).
Antigen Distribution
Henipavirus RBP is an envelope glycoprotein.
Background
Henipavirus spp. are enveloped, single-stranded RNA viruses in the family Paramyxovirus1. Five species have been identified, two of which, Hendra virus (HeV) and Nipah virus (NiV), are highly virulent emerging pathogens with high case-fatality ratios. The other three species, Cedar virus, Ghanaian bat virus, and Mojiang virus are not known to cause human disease. Pteropid bats are the reservoir host. HeV is transmitted by direct contact with infected horses, their fluids, or tissues1. Horses are infected by exposure to pteropid bats. NiV is transmitted by contact with infected pigs or bats and person-to-person. Both HeV and NiV cause severe influenza-like illness that can progress to encephalitis.

Potently neutralizing antibodies to HeV and/or NiV have been identified2, 3, 4, 5. HENV-103 was isolated from circulating B cells of an individual exposed to equine HeV vaccine5. Peripheral blood mononuclear cells were tested for binding to recombinant forms of the receptor binding protein (RBP) of NiVB, NiVM, or HeV. The mAb panel grouped into at least six distinct antigenic sites, A-F. HENV-103, group D, binds recombinant RBP and neutralizes HeV, NiVM, and NiVB.. HENV-103 maps to a distinct site on the HeV-RPB head domain spanning the β1 and β6 propeller blades, a region at the interface between protomers within the dimer-of-dimers structure of the HeV-RBP tetramer, suggesting a semi-cryptic site of vulnerability. nsEM mapping shows that HENV-103 binds at the putative dimeric interface. Binding of HENV-103 to RBP is enhanced by ephrin-B2.

Antigen Details

Research Area
Henipavirus
.
Infectious Disease
.
Viral

References & Citations

1. Aguilar HC, Ataman ZA, Aspericueta V, et al. J Biol Chem. 284(3):1628-1635. 2009.
2. Mire CE, Chan YP, Borisevich V, et al. J Infect Dis. 221(Suppl 4):S471-S479. 2020.
3. Zhu Z, Dimitrov AS, Bossart KN, et al. J Virol. 80(2):891-899. 2006.
4. Doyle MP, Kose N, Borisevich V, et al. Cell Rep. Aug 31;36(9):109628. 2021.
Flow Cytometry

Formats Available

Prod No.
Description
LT575
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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