Anti-HER2 (Phospho-Tyr1248)
Anti-HER2 (Phospho-Tyr1248)
Product No.: 43079
Data
Immunoblotting: Detection of HER2 (phospho-Tyr1248) in extracts of HepG2 cells treated with PMA and MCF cells treated
with EGF.
Immunohistochemistry: Detection of HER2 (phospho-Tyr1248) in paraffin-embedded human breast carcinoma tissue (left) and
human lung carcinoma tissue (right).
Immunofluorescence: Detection of HER2 (phospho-Tyr1248) in methanol-fixed MCF-7 cells. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of tyrosine 1248 (P-E-Y(p)-L-G) derived from human HER2 and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~185kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100. Immunofluorescence: use at dilution of 1:100-1:200. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to HER2 (Phospho-Tyr1248) Background HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years HER2 has become an important biomarker and target of therapy for approximately 30% of breast cancer patients. Antigen DetailsFunction Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. {PubMed:10358079, PubMed:15380516, PubMed:21555369}. NCBI Gene Bank ID UniProt.org Research Area Cancer . Phosphospecific Antibodies References & CitationsTechnical Protocols  |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
