Anti-HNF4α (phospho-Ser304) Antibody (43043)
Anti-HNF4α (phospho-Ser304) Antibody (43043)
Product No.: 43043
Data
Immunoblot: Detection of HNF4α (phospho-Ser304) in extracts of HeLa cells treated or untreated with Anisomycin. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 304 (L-R-S(p)-Q-V) derived from human HNF4a and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:1,000. A band of ~52kDa is detected.
These are recommended working dilutions. End user should determine optimal dilutions for their applications Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to HNF4 α (phospho-Ser304) Background Hepatocyte nuclear factor 4 alpha (HNF4alpha) is a nuclear transcription factor that binds DNA as a homodimer and controls the expression of several genes including hepatocyte nuclear factor 1, a transcripton factor that regulates expression of several hepatic genes. HNF4alpha has been implicated in development of liver, kidney, and intestines. Mutations in the HNF4alpha gene have been associated with monogenic autosomal-dominant non-insulin-dependent diabetes mellitus type 1. Alternative splicing of this gene results in multiple transcript variants. Antigen DetailsFunction Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698). {UniProtKB:P49698, PubMed:30530698, PubMed:30597922}. NCBI Gene Bank ID UniProt.org Research Area Phosphospecific Antibodies References & CitationsTechnical Protocols |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
