Anti-Human Bit1 (NT)

Anti-Human Bit1 (NT)

Product No.: B462

[product_table name="All Top" skus="B462"]

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Target
Bit1
Product Type
Polyclonal Antibody
Alternate Names
Bcl-2 inhibitor of transcription 1, Peptidyl-tRNA hydrolase 2
Applications
IHC FFPE
,
WB

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rabbit
Immunogen
PN:B493
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Bit1 recognizes an epitope near the N-terminus of human, mouse and rat Bit1. This polyclonal antibody was purified using affinity chromatography.
Background
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment.1 Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process.2 Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination.3 It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE.2 Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the α5β1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the α5β1 integrin.2

Antigen Details

PubMed

References & Citations

1. Martin, SS. and Vuori, K. (2004) Biochim Biophys Acta. 1692:145-57.
2. Pai, JT. et al.(2004) Cell 116:751-762.
3. Chen, G. and Courey, AJ. (2000) Gene 249:1-16.
IHC FFPE
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.