This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human CD13 (Clone 22A5) recognizes Human CD13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD13 recognizes the (Mr 150-170 kDa) cell surface glycoprotein expressed in a pan-myeloid fashion. This antibody also reacts with osteoclasts in giant cell tumors of bone (osteoclastoma), clear cell chondrosarcoma and aneurysmal bone cysts.2
The CD13 antigen is present on most cells of myeloid origin, including granulocytes and monocytes in normal peripheral blood. CD13 is not expressed on B-cells, T-cells, platelets or erythrocytes. Expression of this antigen is greater on monocytes than on granulocytes.
Anti-Human CD13 can be used to study myeloid cell functions as well as to immunophenotype leukemias. CD13 is present on basophils from subjects with chronic myeloid leukemia (CML) and on granulocytes from subjects with systemic lupus erythematosus (SLE).
NCBI Gene Bank ID
References & Citations
1. Zaia, JA. et al. (1996) J Infect Dis. 173(5):1062-71. Article Link
2. Horton, A. M. et al. (1985) Cancer Res. 45:5663
Products are for research use only. Not for use in diagnostic or therapeutic procedures.