NS0-Derived Recombinant Human 4-1BB Extracellular Domain (Accession # Q07011)
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing 4-1BB. A suggested method would be to stain cells expressing 4-1BB with 0.25 µg per 1 - 2.5 x 105 cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human 4-1BB this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human 4-1BB is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone 145501 recognizes an epitope on human 4-1BB.
4-1BB is expressed on activated B cells and T cells, macrophages, and dendritic cells.
4-1BB (CD137) is a 39 kD transmembrane protein that is a member of the tumor necrosis factor (TNF) receptor family and is a co-stimulatory molecule that plays a role in T-cell-mediated proliferative response. When binding its ligand, CD137 provides costimulatory signals to both CD4 and CD8 T cells via the activation of NF-B, c-Jun and p38 downstream pathways. Crosslinking of CD137 boosts T cell proliferation, IL-2 secretion, survival and cytolytic activity. Furthermore, it can increase immune activity to eliminate tumors in mice. Agonistic anti-CD137 antibodies have been reported to stimulate a more intense immune system attack on cancers.
NCBI Gene Bank ID
References & Citations
1. Shan, B. et al. (2008) Cell Mol Immunol. 5: 379
2. Kwan, BS. et al. (2009) J Immunol. 182: 4107
3. Kwan, BS. et al. (1998) Eur J Immunol. 28: 881
4. Kwan, BS. et al. (1996) Cell Immunol. 169: 91
Products are for research use only. Not for use in diagnostic or therapeutic procedures.