Anti-Human CD137 (TNFRSF9) (Clone CPTC-TNFRSF9-1) – Purified No Carrier Protein

CD137 synthetic peptide NQICSPCPPNSFSSAGGQR

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

FSAI150-3G10 activity is directed against TNFRSF9 (also known as 4-1BB).

CPTC-TNFRSF9-5

TNFRSF9, also known as 4-1BB (CD137), is a type I transmembrane protein that is a member of the tumor necrosis factor receptor (TNFR) superfamily¹. CD137 functions as a co-stimulatory molecule, enhancing T cell proliferation, survival, and cytokine production upon binding to its ligand TNFSF9 (also known as 4-1BBL or CD137L) on activated antigen-presenting cells. This interaction plays a crucial role in immune responses and protects against cancer. Dysregulation of TNFRSF9 has been observed in pancreatic, lung, and colorectal cancers. As such, TNFRSF9 is a target for cancer immunotherapy, and agonists have been tested for anti-tumor activity² . These agonists improve anti-tumoral T cell activation, proliferation, and survival as well as mitochondrial activity in CD8 T cells. CAR T cell therapies are being investigated in conjunction with the monoclonal agonists.

FSAI150-3G10 was generated in mouse using a synthetic peptide derived from human TNFRSF9 that is carbamidomethylated at both cysteines, NQICSPCPPNSFSSAGGQR 3 . FSAI150-3G10 was developed for use in immuno-MRM assays. FSAI150-3G10 does not detect TNFRSF9 when used to probe Western blots loaded with cell lysates or TNFRSF9 recombinant protein.

TNFRSF9 is expressed on the surface of activated T cells.

TNFSF9 (also known as 4-1BBL)

1 Glorieux C, Huang P. Cancer Communications. 39(1):70. 2019.
2 Claus C, Ferrara-Koller C, Klein C. MAbs. 15(1):2167189. 2023.
3 Whiteaker JR, Lundeen RA, Zhao L, et al. Front Immunol. 12:765898. 2021.