Anti-Human CD163 (Clone CPTC-CD163-1) – Purified No Carrier Protein

CD163 synthetic peptide LVDGVTE(Cam)SGR

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

EB0967B-1G6-H1/K1 activity is directed against human CD163 peptide sequence LVDGVTECSGR.

CPTC-CD163-1

CD163 is a transmembrane protein that acts as an endocytic, hemoglobin scavenger receptor with a role in inflammation¹. CD163 has class B scavenger receptor cysteine-rich (SRCR) domains that allow for molecular interactions, calcium binding, and haptoglobin–hemoglobin (Hp-Hb) complex binding. CD163-positive macrophages in the liver, spleen, and bone marrow bind and remove Hp-Hb complexes from circulation by uptake into the macrophage, where heme is degraded by heme oxygenase-1 and anti-inflammatory metabolites Fe²⁺, CO, and biliverdin/bilirubin are produced. This allows for both the recycling of iron and an anti- inflammatory response that includes inhibition of proinflammatory cytokines, lipid/protein peroxidation, complement cascade, and attenuation of leukocyte rolling. CD163-expressing macrophages are found at sites of inflammation and atherosclerotic plaques, where their presence is protective, as well as in the tumor microenvironment. CD163 also functions as a sensor for the recognition of Streptococcus mutans, Eschericia coli, and Staphylococcus aureus and promotes phagocytosis and killing of the bacteria. CD163-expressing macrophages are being developed as delivery vehicles for anti-inflammatory and tumoricidal drugs².

EB0967B-1G6-H1/K1 was generated in rabbit for use in immuno-MRM assays using a synthetic peptide derived from human CD163 that is carbamidomethylated at the cysteine, VDGVTEC[cam]SGR³. EB0967B-1G6-H1/K1 does not detect CD163 by immunohistochemistry⁴.

https://antibodies.cancer.gov/detail/CPTC-CD163-1#CPTC-CD163-1

CD163 is expressed on monocytes and mature macrophages (e.g., Kupffer cells, red pulp, cortical, lung, resident bone marrow, meningeal). A soluble CD163 isoform can also be released into the plasma, cerebral spinal fluid, and urine by proteolytic shedding. Soluble CD163 increases in acute and chronic inflammations.

CSNK2B, hemoglobin/haptoglobin complexes, TWEAK

1 Etzerodt A, Moestrup SK. Antioxid Redox Signal. 18(17):2352-2363. 2013.
2 Skytthe MK, Graversen JH, Moestrup SK. Int J Mol Sci. 21(15):5497. 2020.
3 Whiteaker JR, Lundeen RA, Zhao L, et al. Front Immunol. 12:765898. 2021.
4 https://antibodies.cancer.gov/detail/CPTC-CD33-1