Anti-Human CD20

Pricing & Details

Product No.M1343
Clone
396444
Protein
CD20
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
MS4A
Isotype
Mouse
IgG1
Applications
CyTOF®
,
FC
,
IHC
Prod No.
Size
Price
Avail.
Qty
Add to cart
M1343-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
M1343-100 µg
100 µg
$339.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human MS4A (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MS4A. A suggested method would be to stain cells expressing MS4A with 25 µg per 1-2.5 x 105 cells in a total staining volume of 200 µl followed by PN:M1189.
Other Applications Reported In Literature ?
Immunohistochemistry: 5-25 µg/mL
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 396444 recognizes an epitope on human CD20.
Antigen Distribution
CD20 is present on human pre B lymphocytes and on B lymphocytes, except on plasma cells, and is expressed in tandem with surface IgM. It is also expressed in some follicular dendritic cells and at low levels on a T cell subset.
Background
CD20 is a 33-37 kD transmembrane-spanning phosphoprotein that facilitates optimal B-cell immune response against T-independent antigens. It can exist in a complex with MHC class I, MHC class II, CD53, CD81, and CD82. However, CD20 can also form homo-oligomers. It has been suggested that homo-oligomerization of CD20 forms calcium ion channels in the plasma membrane of B cells. CD20 can be useful in diagnosing B-cell lymphomas and leukemias, and is the target of mAbs in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases. Interestingly, studies show a link between the immune system's B cells and diabetes mellitus in which anti-CD20 antibodies rendered the T cell antibodies dysfunctional and, hence, unable to cause insulin desensitivity by a B cell antibody-modulated autoimmune response.

Antigen Details

Protein
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Function
B cell activation
PubMed
NCBI Gene Bank ID
Flow Cytometry
IHC

Formats Available

Prod No.
Description
M1343
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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