Anti-Human CD20 – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C1652
Clone
2H7
Protein
CD20
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
MS4A1, B1, Bp35
Isotype
Mouse
IgG2b κ
Applications
CyTOF®
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1652-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
C1652-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
C1652-25 mg
25 mg
$800.00
In stock
Max:
Min: 1
Step: 1
C1652-50 mg
50 mg
$1,325.00
In stock
Max:
Min: 1
Step: 1
C1652-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human tonsillar B cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 2H7 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this 2H7 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CyTOF®
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 2H7 recognizes an epitope on human CD20.
Antigen Distribution
CD20 is present on human pre B lymphocytes and on B lymphocytes, except on plasma cells, and is expressed in tandem with surface IgM. It is also expressed in some follicular dendritic cells and at low levels on a T cell subset.
Background
CD20 is a 33-37 kD transmembrane-spanning phosphoprotein that facilitates optimal B-cell immune response against T-independent antigens. It can exist in a complex with MHC class I, MHC class II, CD53, CD81, and CD82. However, CD20 can also form homo-oligomers. It has been suggested that homo-oligomerization of CD20 forms calcium ion channels in the plasma membrane of B cells. CD20 can be useful in diagnosing B-cell lymphomas and leukemias, and is the target of mAbs in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases. Interestingly, studies show a link between the immune system's B cells and diabetes mellitus in which anti-CD20 antibodies rendered the T cell antibodies dysfunctional and, hence, unable to cause insulin desensitivity by a B cell antibody-modulated autoimmune response.

Antigen Details

Protein
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Function
B cell activation
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules

References & Citations

1. Stashenko, P. et al. (1980) J. Immunol. 125:1678 2. Fang, D. et al. (2005) Cancer Res. 65:9328 3. Bubien, J. K. et al. (1993) J. Cell Biol. 121:1121 4. White, M. W. et al. (1991) J. Immunol. 146:846 5. Cragg, M. S. et al. (2005) Curr. Dir. Autoimmun. 8:140
Flow Cytometry
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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