Anti-Human CD20 – Purified

Anti-Human CD20 – Purified

Product No.: C1602

[product_table name="All Top" skus="C1602"]

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Clone
2H7
Target
CD20
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
MS4A1, B1, Bp35
Isotype
IgG2b κ
Applications
FC
,
IHC FF
,
IP

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CD20 (>98%)
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 2H7 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IHC (Frozen) The suggested concentration for this 2h7 antibody in IHC staining on frozen tissue is ≤ 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone 2H7 recognizes an epitope on human CD20.
Background
CD20 is a 33-37 kD transmembrane-spanning phosphoprotein that facilitates optimal B-cell immune response against T-independent antigens. It can exist in a complex with MHC class I, MHC class II, CD53, CD81, and CD82. However, CD20 can also form homo-oligomers. It has been suggested that homo-oligomerization of CD20 forms calcium ion channels in the plasma membrane of B cells. CD20 can be useful in diagnosing B-cell lymphomas and leukaemias, and is the target of mAbs in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases. Interestingly, studies show a link between the immune system's B cells and diabetes mellitus in which anti-CD20 antibodies rendered the T cell antibodies dysfunctional and, hence, unable to cause insulin desensitivity by a B cell antibody-modulated autoimmune response.
Antigen Distribution
CD20 is present on human pre B lymphocytes and on B lymphocytes, except on plasma cells, and is expressed in tandem with surface IgM. It is also expressed in some follicular dendritic cells and at low levels on a T cell subset.
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1. Stashenko, P. et al. (1980) J. Immunol. 125:1678 2. Fang, D. et al. (2005) Cancer Res. 65:9328 3. Bubien, J. K. et al. (1993) J. Cell Biol. 121:1121 4. White, M. W. et al. (1991) J. Immunol. 146:846 5. Cragg, M. S. et al. (2005) Curr. Dir. Autoimmun. 8:140
Flow Cytometry
IHC FF
Immunoprecipitation Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.