Anti-Human CD25 (IL-2Rα) [Clone 7G7B6] — Purified in vivo PLATINUM™ Functional Grade

Clone 7G7B6 is used in in vivo mouse studies primarily as an anti-human CD25 monoclonal antibody for radioimmunotherapy, targeting CD25-expressing cells such as those found in certain lymphomas. It is typically conjugated with a radioactive isotope (e.g., ^90Y or ^111In) and administered to tumor-bearing mice to assess therapeutic efficacy and biodistribution.

Key context and details:

• Mouse Models: Nude mice are injected subcutaneously with CD25-expressing lymphoma cells (e.g., SUDHL-1) to establish tumors. The antibody is then administered to evaluate effects on tumor growth and animal survival.
• Radioimmunotherapy: ^90Y-labeled 7G7B6 is used to treat established tumors, significantly prolonging mouse survival and clearing tumors in a notable fraction of animals compared to controls.
• Biodistribution: ^111In-labeled antibody is employed to examine the distribution of the antibody in different mouse tissues, supporting the targeting specificity.
• Mechanism: The antibody binds specifically to human CD25 (IL-2R alpha), which is expressed on activated lymphocytes. This allows assessment of therapeutic and biological effects of CD25 targeting in vivo.
• Additional Uses: The antibody is also applied for bioanalytical assays in vivo, such as pharmacokinetic (PK) and anti-drug antibody (ADA) analysis relating to biological pathways affected by CD25.
• Preparation: For in vivo use, the antibody is produced with low endotoxin levels and purified via affinity chromatography, ensuring suitability and safety for animal studies.

In summary, clone 7G7B6 is utilized in mouse studies to investigate targeted radioimmunotherapy against human CD25, evaluate immune modulation, and support analytical assays involving CD25 biology.

The correct storage temperature for the sterile packaged clone 7G7B6 (anti-human CD25 monoclonal antibody) depends on the intended storage duration:

• Short-term storage (up to one month): Store at 2–8°C (refrigerator temperature).
• Medium-term storage (up to 6 months): Some suppliers specify 4°C as stable for up to 6 months.
• Long-term storage: Aseptically aliquot without diluting and store at –20°C or ? –70°C or –80°C for maximal stability.

Additional important notes:

• Avoid repeated freeze-thaw cycles to preserve antibody integrity.
• For the best long-term stability and for functional-grade antibody fractions, storage at –70°C or below is recommended.
• Always refer to the specific supplier’s datasheet for your product lot.

Summary Table:

These guidelines are consistent across reputable suppliers of the 7G7B6 clone.

Commonly used antibodies or proteins alongside 7G7B6 (anti-human CD25, IL-2R?) in the literature include other monoclonal antibodies targeting CD25, components of the IL-2 receptor complex (such as CD122 and CD132), and markers relevant for T cell phenotyping and signaling studies.

Key antibodies and proteins frequently used with 7G7B6:

• BC96 (anti-human CD25): Another monoclonal antibody recognizing a different epitope on CD25, useful for comparative studies or multi-epitope detection.
• CCR7 antibodies: Used for studying signaling complexes involving CD25 and CCR7, especially in migration and activation studies. Both polyclonal and monoclonal anti-CCR7 antibodies are cited.
• Anti-HA (hemagglutinin) antibodies: Employed in studies where tagged receptor subunits are expressed or immunoprecipitated; both mouse and rabbit clones are used for detection.
• Other anti-CD25 clones: Including MA251 and 7D4 (for mouse CD25), commonly used for cross-species comparative immunology.
• Isotype controls or anti-mouse CD25 (e.g., PC61.5.3): Used in experiments with murine cells or for negative controls in human cell assays.
• Secondary antibodies: Such as IRDye-conjugated anti-mouse IgG, for detection in Western blot or immunofluorescence.
• Proteins/ligands: IL-2 itself is often used in functional assays, since CD25 forms part of the high-affinity IL-2 receptor and is critical for IL-2 signaling and T cell proliferation.

Additional commonly used markers in immunology panels:

• CD4, CD8, CD3: For T cell subset delineation.
• CD127 (IL-7R?): Sometimes paired with CD25 for defining regulatory T cells (Tregs).
• FOXP3: Intracellular marker to further specify Tregs in combination with CD25.

These combinations allow researchers to:

• Assess activation states of lymphocytes.
• Study the composition and function of T cell subpopulations, especially regulatory T cells.
• Map protein-protein interactions and downstream signaling pathways associated with CD25.

When using 7G7B6, antibody panels are typically customized to the specific research question, such as immunophenotyping, receptor signaling analysis, or cell depletion protocols. If you need panels for a specific context (e.g., flow cytometry of Tregs, immunoprecipitation, or signaling), please clarify for more detailed combinations.

The key findings from scientific literature citing clone 7G7B6 (an anti-CD25 monoclonal antibody, also written as 7G7/B6) revolve around its specificity, biological effects, and potential therapeutic applications:

• Target specificity and immunoreactivity: 7G7B6 is a monoclonal antibody targeting human CD25 (the alpha chain of the interleukin-2 receptor, IL2RA), which is expressed on activated T cells, some B cells, thymocytes, myeloid precursors, and certain other immune cells. The antibody retains high specificity and immunoreactivity after radiolabeling; over 80% of ^90Y-7G7/B6 and ^111In-7G7/B6 bound to CD25-positive cells, and this binding was specifically blocked by excess unlabelled 7G7/B6.

• Therapeutic and investigational use: Radiolabeled 7G7B6 (notably with ^90Y) has been evaluated as a potential radioimmunotherapeutic agent, showing promise for treating CD25-expressing lymphomas in preclinical models. The antibody preserves its binding capacity after radiolabeling, supporting its relevance for in vivo imaging or therapy.

• Internalization and modulation: 7G7B6 is internalized by CD25-positive cell lines and can modulate CD25 surface expression, as demonstrated by both flow cytometry and radiolabel tracing techniques.

• Immunological roles: CD25 (the antibody target) plays roles in immune tolerance and T cell effector function, with differential importance across species. In humans, CD25 seems more involved in T cell effector responses, while in mice, it is critical for regulatory T cell (Treg) maintenance and immune homeostasis.

• Utility in research applications: 7G7B6 is widely used for flow cytometry (FACS) and other research methods to identify or deplete CD25-expressing cells, helping study immune regulation, lymphoma biology, or antibody-based therapy mechanisms.

• Species reactivity and molecular details: 7G7B6 is a mouse IgG2a kappa monoclonal antibody generated against recombinant human CD25. Its specificity makes it valuable for human immune cell profiling and preclinical therapeutic investigations.

In summary, 7G7B6 is a well-characterized tool for targeting human CD25, enabling both basic immunology research and preclinical therapeutic studies, notably for CD25-positive lymphoid malignancies.