Anti-Human CD28 (Clone 9.3) – Purified No Carrier Protein

Anti-Human CD28 (Clone 9.3) – Purified No Carrier Protein

Product No.: C883

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Product No.C883
Clone
9.3
Target
CD28
Product Type
Recombinant Monoclonal Antibody
Alternate Names
Tp44
Isotype
Mouse IgG2a k
Applications
ELISA
,
FA
,
IF
,
IP

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Expression Host
HEK-293 Cells
Immunogen
Human T lymphocytes
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
ELISA,
FA,
IF,
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
The Anti-Human CD28 Mouse IgG2a, Clone 9.3 antibody, targets human CD28
Antigen Distribution
CD28 is primarily expressed by thymocytes, most peripheral T cells, and natural killer (NK) cells.
Background
CD28, a glycoprotein weighing 44 kDa is a member of the Ig superfamily and plays a vital role, in the activation and survival of T cells1, 2 by enhancing the production of interleukin and the activity of T cells. CD28 is unique among B7 receptors as it is consistently expressed on T cells. The interaction between CD28 and B7 is crucial for preventing T cells from becoming anergic and unable to respond to antigen/MHC complexes highlighting its role in an immune response3-6.

The clone 9.3 has gained recognition for its ability to stimulate the growth of human T cells in vitro making it a valuable tool for research focused on studying T cell activation and immune responses. It was developed by immunizing mice with human T cells. It has demonstrated effectiveness in inducing T cell activation and proliferation when combined with TPA thereby influencing IL 2 receptor expression and IL 2 production7. This clone can be utilized for staining a portion of T cells through immunofluorescence methods and has proven successful in immunoprecipitating CD28 from Jurkat cells8. Given its ability to activate T cells and its reactivity with CD28, the 9.3 clone stands as a resource for research.

Antigen Details

Ligand/Receptor
CD80 (B7-1) and CD86 (B7-2)
NCBI Gene Bank ID
UniProt.org
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1. Porciello N, Tuosto L. Cytokine Growth Factor Rev. 2016;28:11-19.
2. Xia S, Chen Q, Niu B. Curr Drug Targets. 2020;21(6):589-598.
3. Eck SC, Chang D, Wells AD, Turka LA. Transplantation. 1997;64(10):1497-1499.
4. Linsley PS, Brady W, Grosmaire L, Aruffo A, Damle NK, Ledbetter JA. J Exp Med. 1991;173(3):721-730.
5. Wu Y, Guo Y, Huang A, Zheng P, Liu Y. J Exp Med. 1997;185(7):1327-1335.
6. Sperling AI, Auger JA, Ehst BD, Rulifson IC, Thompson CB, Bluestone JA. J Immunol.1996;157(9):3909-3917.
7. Baroja ML, Lorre K, Van Vaeck F, Ceuppens JL. Cell Immunol. 1989;120(1):205-217.
8. Hara T, Fu SM, Hansen JA. Human T cell activation. II. J Exp Med. 1985;161(6):1513-1524.
9. Kovacs B, Parry RV, Ma Z, et al. J Immunol. 2005;175(12):7848-7854.
10. Bushkin Y, Radford F, Pine R, et al. J Immunol. 2015;194(2):836-841.
11. Hill EV, Ng THS, Burton BR, Oakley CM, Malik K, Wraith DC. Eur J Immunol. 2015;45(4):1103-1115.
12. Huang X, Williams JZ, Chang R, et al. Nat Nanotechnol. 2021;16(2):214-223.
13. Siefken R, Klein-Hessling S, Serfling E, Kurrle R, Schwinzer R. J Immunol. 1998;161(4):1645-1651.
Indirect Elisa Protocol
FA
IF
Immunoprecipitation Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.