Anti-Human CD4 (Ibalizumab)

Product No.: LT3200

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Product No.LT3200
Product Type
Biosimilar Recombinant Human Monoclonal Antibody
Alternate Names
CD4, CD4mut, CD4 molecule, OKT4D, IMD79
Human IgG4κ

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Antibody Details

Product Details

Reactive Species
Expression Host
HEK-293 Cells
FC Effector Activity
Recommended Isotype Controls
Recombinant Human CD4
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
≥95% by SDS Page
≥95% monomer by analytical SEC
This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Product Preparation
Recombinant biosimilar antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only (RUO). Non-Therapeutic.
Country of Origin
2-8°C Wet Ice
Applications and Recommended Usage?
Quality Tested by Leinco
Additional Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Ibalizumab binds to domain 2 of CD4 T cell receptors, on the protein surface opposite where the major histocompatibility complex-class II and HIV-1 gp120 binding sites are located. Ibalizumab binds to both human and monkey CD4.
Antigen Distribution
CD4 is primarily found on T lymphocytes.
CD4 is a cell surface glycoprotein essential for both T cell activation and human immunodeficiency virus type-1 (HIV-1) infection 1,2. CD4 consists of an extracellular segment composed of four tandem immunoglobulin-like domains (D1 to D4), a single transmembrane span, and a short C-terminal cytoplasmic tail 1. HIV-1 entry into host CD4 cells is a complex process that occurs through the interaction of HIV-1 glycoprotein 120 (gp120) with extracellular CD4 D1 3,4. When gp120 binds to CD4, a conformational shift occurs that allows co-receptors to bind to the gp120/receptor complex, leading to viral fusion and entry. Ibalizumab is the first CD4-directed post-attachment HIV-1 entry inhibitor and prevents entry of a broad spectrum of HIV-1 isolates 1,5,6,7,8.

Ibalizumab selectively binds to an epitope on CD4 D2 (residues 121-124 and 127-134 9 and especially L96, P121, P122, and Q163 1,7) as well as residues E77 and S79 on D1 at the interface between D1 and D2 4,7. Ibalizumab primarily contacts the BC-loop in D2 at the D1-D2 junction on the opposite side to the gp120 and major histocompatibility complex II (MHC-II) binding sites 2. Ibalizumab does not inhibit HIV-1 gp120 binding to D1, but instead induces conformational changes that via steric hindrance block gp120 and HIV co-receptors from interacting, thereby preventing viral fusion and entry 3,4,7,10,11. Additionally, because the cellular epitope is distant from the D1 MHC-II binding site 4, MHC-II mediated immunosuppression is prevented 3. Furthermore, as a humanized IgG4 antibody, ibalizumab displays low affinity for C1q and FcɣRI receptors of natural killer cells and consequently has low cellular cytotoxic dependent activity and no Fc-mediated CD4+ T cell depletion 3.

Ibalizumab was derived from mu5A8 by grafting the mouse complementary-determining region onto a human IgG4 construct 1,3,12. The chemical name is immunoglobulin G4, anti-(human CD4 (antigen)) (human-mouse monoclonal 5A8 γ4-chain), disulphide with human-mouse monoclonal 5A8 κ-chain, dimer 5.

Antigen Details

CD4/CD4 receptor
NCBI Gene Bank ID
Research Area

References & Citations

1. Song R, Franco D, Kao CY, et al. J Virol. 84(14):6935–6942. 2010.
2. Freeman MM, Seaman MS, Rits-Volloch S, et al. Structure. 18(12):1632–1641. 2010.
3. Iacob SA, Iacob DG. Front Microbiol. 8:2323. 2017.
4. Chahine EB, Durham SH. Ann Pharmacother. 55(2):230-239. 2021.
5. Markham A. Drugs. 78(7):781-785. 2018.
6. Reimann KA, Lin W, Bixler S, et al. AIDS Res Hum Retroviruses. 13(11):933-943. 1997.
7. Beccari MV, Mogle BT, Sidman EF, et al. Antimicrob Agents Chemother. 63(6):e00110-19. 2019.
8. Blair HA. Drugs. 80(2):189-196. 2020.
9. Burkly LC, Olson D, Shapiro R, et al. J Immunol. 149:1779–1787. 1992.
10. Moore JP, Sattentau QJ, Klasse PJ, et al. J Virol. 66(8):4784-4793. 1992.
11. Bettiker RL, Koren DE, Jacobson JM. Curr Opin HIV AIDS. 13(4):354-358. 2018.
12. Boon L, Holland B, Gordon W, et al. Toxicology. 172(3):191-203. 2002.
13. Reimann KA, Khunkhun R, Lin W, et al. AIDS Res Hum Retroviruses. 18(11):747-755. 2002.
14. Kuritzkes DR, Jacobson J, Powderly WG, et al. J Infect Dis. 189(2):286-291. 2004.
15. Jacobson JM, Kuritzkes DR, Godofsky E, et al. Antimicrob Agents Chemother. 53(2):450-457. 2009.
16. Toma J, Weinheimer SP, Stawiski E, et al. J Virol. 85(8):3872–3880. 2011.
17. Pace CS, Fordyce MW, Franco D, et al. J Acquir Immune Defic Syndr. 62(1):1–9. 2013.
Indirect Elisa Protocol
Flow Cytometry
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.