Anti-Human CD40 (Clone HB-14) – Purified in vivo GOLD^TM Functional Grade

The antibody HB-14 (clone name) is an anti-CD40 monoclonal antibody widely used in in vivo mouse studies to modulate immune responses. CD40 is a costimulatory protein found mainly on antigen-presenting cells, and engaging or blocking this molecule can profoundly affect immune function.

In vivo Uses of Clone HB-14:

• Activation or Blockade of CD40: In mouse models, HB-14 is used to activate or block the CD40 receptor on dendritic cells and other antigen-presenting cells, thus influencing T cell-dependent immune responses.
• Study of Immune Pathways: It is applied to investigate the role of CD40-mediated signaling pathways, including their effects on dendritic cell maturation, cytokine production, B cell help, and immune regulation.
• Phenotype Analysis: This antibody has also been used for ex vivo and in vivo characterization of immune cell populations by flow cytometry, particularly for dendritic cell (DC) phenotype analysis.

Experimental Applications:

• Flow Cytometry: To identify and analyze CD40+ cells within various tissues or blood of mice after treatment or in disease models.
• Immune Modulation: Administration of HB-14 in mice (by injection) to enhance or inhibit immune reactions in cancer models, transplantation, or during infection, usually by targeting CD40-expressing cells.

Typical Protocols and Considerations:

• The antibody can be injected intraperitoneally or intravenously, but the optimal dosing and frequency depend on the experiment's goal and must be determined experimentally.
• HB-14 can be used as a functional-grade antibody, meaning it is suitable for use in live animal experiments without causing unwanted effector functions due to its Fc region modifications or isotype selection.
• Effects on immune modulation can include enhanced antigen presentation, T cell priming, and/or B cell activation, depending on whether the antibody acts as an agonist (activates CD40 signaling) or antagonist (blocks ligand binding).

Summary Table: HB-14 in Mouse Studies

For individual protocols and recommended dilutions or dosing, consult the antibody datasheet or primary research articles using HB-14.

Note: If you meant a different HB-14 (not anti-CD40), please specify the target antigen or context for clarification. Different antibodies and clones may share similar nomenclature. The above details are based on anti-CD40 [HB-14] as listed in the provided sources.

To determine the correct storage temperature for the sterile packaged clone HB-14, we would need specific information about the product itself, such as its composition or intended use. However, based on common practices for storing biological materials and sterile products, here are some general guidelines:

1. General Storage for Biological Reagents: Many biological reagents, including some types of clones, are typically stored at freezer temperatures (e.g., -20°C or -80°C) to maintain stability and prevent degradation. However, specific storage temperatures can vary based on the product's formulation and stability characteristics.

2. Reference to QIAprep Miniprep Kits: These kits are stored dry at room temperature (15–25°C) for up to 12 months but can also be stored at 2–8°C for longer periods or special conditions.

3. Vaccine Storage: Vaccines, like RECOMBIVAX HB or GARDASIL 9, are stored refrigerated at 2°C to 8°C to maintain potency.

Without specific details about the clone HB-14, it is difficult to provide precise storage instructions. Typically, if the product is a biological clone (e.g., a genetic material), it might be stored frozen to preserve integrity, but this would depend on the clone's characteristics and handling guidelines.

For precise storage conditions, refer to the product documentation or contact the manufacturer or supplier for specific guidance.

Commonly used antibodies and proteins with HB-14 (anti-human CD40 antibody) in the literature typically include those targeting B cell markers, activation molecules, and cytokines, as well as reagents for costimulatory cell signaling and immune cell characterization.

Key antibodies/proteins frequently used with HB-14 in experimental setups include:

• Anti-IgM: Frequently combined with HB-14 to promote B cell proliferation or activation, highlighting co-stimulation mechanisms.
• Interleukin-4 (IL-4): Often used alongside HB-14 to support B cell proliferation and differentiation tasks.
• Phorbol 12-myristate 13-acetate (PMA): Utilized in combination with HB-14 for robust B cell activation studies.
• TLR ligands (e.g., CpG, LPS): Used to stimulate regulatory B10 cell production or immune responses in conjunction with HB-14, particularly for studying cytokine secretion such as IL-10.
• CD40L (CD154): As the natural ligand for CD40, it is central in studies examining HB-14-mediated signaling, cell-to-cell interactions, and rescue from apoptosis.
• Other B cell surface markers (such as CD19, CD20, or CD86): These are commonly used in multicolor flow cytometry panels with HB-14 to distinguish B cell subsets or activation states.

Associated reagents may also include:

• Endotoxin-free buffers and azide-free formulations, necessary for cell culture and functional assays using HB-14.
• Various fluorochrome-conjugated HB-14 formats (e.g., FITC, PE, APC), allowing multiplex analysis with other antibodies targeting distinct cell markers.

The literature routinely references combinations of HB-14 with anti-IgM, cytokines (e.g., IL-4), and TLR agonists, indicating their roles in dissecting B cell regulation, costimulation, and differentiation pathways in immunology research.

The search results do not contain specific key findings from clone HB-14 citations in scientific literature. The mention of clone HB-14 appears in the context of a scientific publication without providing details about the clone itself or its findings. If you are looking for specific research or findings related to clone HB-14, you may need to access the full text of the relevant publication or search through broader scientific databases like PubMed or Google Scholar. However, here are some related areas of research:

Related Research Areas

1. Immunology and Cell Biology: Research involving clones like HB-14 often focuses on understanding cell biology mechanisms, which can include studies on cell signaling, gene expression, or immune responses.

2. Base Editing and Gene Regulation: Recent studies have explored the use of base editing to modify gene regulation, particularly in the context of globin gene expression and fetal hemoglobin (HbF) induction.

3. BCL11A and Hemoglobinopathies: The role of BCL11A in regulating ?-globin and its potential as a therapeutic target for hemoglobinopathies is an active area of research.

Without specific details on clone HB-14, it is challenging to pinpoint key findings directly related to this clone. If you have more context or details, further targeted searches might yield more relevant results.