Anti-Human CD45 (Clone 2D1) – Biotin
Anti-Human CD45 (Clone 2D1) – Biotin
Product No.: C892
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Clone 2D1 Target CD45 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names CD45 Isotype Mouse IgG1 κ Applications FC , IHC |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Human PBMC Product Concentration 0.5 mg/ml Formulation This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. State of Matter Liquid Storage and Handling This biotinylated antibody is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? FC IHC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 2D1 activity is directed against human CD45.
Background CD45 is a receptor protein tyrosine phosphatase involved in immune cell activation1. It is a large
glycoprotein (180-220 kDa) with a broad range of expression in the animal kingdom, including
mammals, chicken, shark, and mosquitoes. The cytoplasmic region of CD45 is highly conserved
in mammals but its extracellular region varies between species. Additionally, differential splicing
leads to several CD45 isoforms which can vary in glycosylation, which in turn can affect CD45
ligand interaction and function. In T cells, isoform expression is dependent on the stage of T cell
maturation, activation, and differentiation. CD45 functions in the immunological synapse of the T cell-antigen presenting cell contact zone that is formed when T cells encounter cognate antigens presented by major histocompatibility (MHC) molecules 1. CD45 interacts with Src kinases, in particular Lck, to mediate antigen receptor signaling in T and B cells, and is required for the development and activation of lymphocytes. CD45 also interacts with CD3ζ and CD3ε chains as well as tyrosine kinase Zap 70, DAP12, the Janus kinase family, PAG, CD22, among others. With these interactions, CD45 functions to protect the host against viral, bacterial, and fungal infections. Certain variants of CD45 are associated with autoimmune disease and dysregulation is observed in some cancers 1. CD45 has been explored as a target of therapeutic modulation for organ transplantation 1, 2 , autoimmune disease 1, bone marrow transfusion 3, 4, 5, 6 as well as cancer radioimmunotherapy 7, 8, including a bispecific antibody targeting CD45 and 90 Y-DOTA for therapy of myeloid leukemia 9. 2D1 was generated by immunizing BALB/c mice with human peripheral blood mononuclear cells 10, 11. Spleen cells were fused to the P3/NSI/I-Ag4-1 myeloma line and resulting supernatants screened by an indirect I125 labeled antiglobulin binding assay. CD45 is also known as leukocyte common antigen (LCA), lymphocyte antigen 5 (Ly-5), and T200 12. 2D1 is also known as anti-HLe-1 10, 11. Antigen Distribution CD45 is expressed on the surface of all nucleated hematopoietic cells and their precursors, except mature erythrocytes and platelets.
Ligand/Receptor Galectin-1, CD2, CD3, and CD4 NCBI Gene Bank ID UniProt.org Research Area Biomarker . Cancer . Immunology References & Citations1 Rheinländer A, Schraven B, Bommhardt U. Immunol Lett. 196:22-32. 2018. 2 Orozco JJ, Kenoyer A, Balkin ER, et al. Blood. 127(3):352-359. 2016. 3 Ruffner KL, Martin PJ, Hussell S, et al. Cancer Res. 61(13):5126-5131. 2001. 4 Matthews DC, Badger CC, Fisher DR, et al. Cancer Res. 52(5):1228-1234. 1992. 5 Matthews DC, Martin PJ, Nourigat C, et al. Blood. 93(2):737-745. 1999. 6 Koronyo-Hamaoui M, Sheyn J, Hayden EY, et al. Brain. 143(1):336-358. 2020. 7 Orozco JJ, Balkin ER, Gooley TA, et al. PLoS One. 9(12):e113601. 2014. 8 Dawicki W, Allen KJH, Garg R, et al. Oncotarget. 11(39):3571-3581. 2020. 9 Orozco JJ, Kenoyer AL, Lin Y, et al. Mol Cancer Ther. 19(12):2575-2584. 2020. 10 Bev80 Beverley PC, Linch D, Delia D. Nature. 287(5780):332-333. 1980. 11 Beverley PCL, Linch D, Callard RE. Human Leucocyte Antigens. In: Neth R, Gallo RC, Graf T, Mannweiler K, Winkler K. (eds) Modern Trends in Human Leukemia IV. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 26. Springer, Berlin, Heidelberg. 1981. 12 https://www.uniprot.org/uniprotkb/P06800/entry 13 Wellhausen SR, Slone SP, Miller JJ. Cytometry B Clin Cytom. 72(5):423-426. 2007. 14 Nathalang O, Siriphanthong K, Petvises S, et al. Ann Lab Med. 38(4):362-366. 2018. 15 Oyer JL, Croom-Perez TJ, Dieffenthaller TA, et al. Front Immunol. 13:861681. 2022. 16 Csiba A, Whitwell HL, Moore M. Br J Cancer. 50(5):699-709. 1984. 17 Terry LA, Brown MH, Beverley PC. Immunology. 64(2):331-336. 1988. Technical ProtocolsCertificate of Analysis |
Formats Available
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Prod No. | Description |
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C890 | |
C892 | |
C895 | |
C893 | |
C894 | |
C972 | |
C896 | |
C897 | |
C898 | |
C899 |
