Anti-Human CD45 (Clone T29/33) – Purified

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Pricing & Details

Product No.C125
Clone
T29/33
Protein
CD45
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
LCA [Leukocyte Common Antigen]
Isotype
Mouse
IgG2b
Applications
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C125-50 µg
50 µg
$55.00
In stock
Max:
Min: 1
Step: 1
C125-100 µg
100 µg
$93.00
In stock
Max:
Min: 1
Step: 1
C125-200 µg
200 µg
$165.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone T29/33 recognizes an epitope on human CD45.
Antigen Distribution
The CD45 antigen is present on all human leukocytes and progenitors in bone marrow. This antibody binds to all human hematopoietic cells, but not to non-hematopoietic cells. Anti-CD45 (T29/33) is not cytotoxic in the presence of complement.
Background
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A,B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.

Antigen Details

Protein
Ligand/Receptor
Galectin-1, CD2, CD3, and CD4.
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Inhibitory Molecules
.
Neuroscience
.
Neuroscience Cell Markers

References & Citations

1. Trowbridge, I. S. et al. (1980) J. Exp. Med. 152:842
2. Battifora, H. et al. (1983) Cancer 51:816
3. Borowitz, M. J. et al. (1983) Human Pathology 15:928
Flow Cytometry

Formats Available