Anti-Mouse CD45 (Clone I3/2.3) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2843
Clone
I3/2.3
Protein
CD45
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
T200, Ly-5, LCA
Isotype
Rat
IgG2b
Applications
FC
,
IHC FF
,
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2843-1.0 mg
1.0 mg
$98.00
In stock
Max:
Min: 1
Step: 1
C2843-5.0 mg
5.0 mg
$325.00
In stock
Max:
Min: 1
Step: 1
C2843-25 mg
25 mg
$875.00
In stock
Max:
Min: 1
Step: 1
C2843-50 mg
50 mg
$1,450.00
In stock
Max:
Min: 1
Step: 1
C2843-100 mg
100 mg
$2,125.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Mouse lymphoma cell line
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this I3/2.3 antibody for staining cells in flow cytometry is ≤ 0.125 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this I3/2.3 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
IHC (Frozen)
IHC (Paraffin)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone I3/2.3 recognizes an epitope on mouse CD45.
Antigen Distribution
CD45 is expressed on all hematopoietic cells other than mature erythrocytes and platelets.
Background
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.

Antigen Details

Protein
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Function
Phosphatase, T and B cell activation
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Inhibitory Molecules
.
Neuroscience
.
Neuroscience Cell Markers
.
Stem Cell
Flow Cytometry
IHC
IHC
General Western Blot Protocol