Anti-Human CD64 (FCGR1) – Purified in vivo PLATINUM™ Functional Grade

In in vivo mouse studies, clone 10.1 is primarily used as a monoclonal antibody against the human CD64 (Fc gamma receptor I) antigen for detection, functional blocking, or depletion experiments. However, its direct use in standard wild-type mouse models is limited because clone 10.1 recognizes human CD64, not native mouse CD64.

Typical Uses of Clone 10.1 in Mouse Studies

• Humanized Mouse Models: Clone 10.1 is used in in vivo experiments involving mice engineered to express human CD64, such as mice with human immune cell engraftment or knock-in lines expressing the human Fc?RI gene. In these cases, clone 10.1 can bind to human CD64 on myeloid cells to:

  • Block IgG binding to Fc?RI, interfering with antibody-dependent cellular functions.
  • Deplete CD64+ cells (e.g., monocytes/macrophages) via antibody-mediated clearance, when administered in vivo and suitably formulated.
  • Track or image human CD64+ cells using tagged versions of the antibody (e.g., fluorophore or radioisotope conjugates) for flow cytometry or imaging studies.

• Transgenic/Knock-In Strains: Recent advances allow usage in mice genetically engineered to express human IgG1 and Fc?Rs (such as Fc?RI/CD64), enabling more physiologically relevant testing of human antibody therapies and Fc?R interactions. Clone 10.1 can be administered in vivo to probe receptor function, antibody clearance, and immune modulation in these settings.

Key Features and Considerations

• Specificity: Clone 10.1 specifically recognizes the human CD64 antigen, with some cross-reactivity to related primate species but not native mouse CD64.
• Functional Activity: The antibody blocks IgG binding to Fc?RI, thereby inhibiting downstream Fc-mediated immune responses (e.g., phagocytosis, cytokine release).
• Research Applications:
  • Analysis and modulation of innate immune responses in humanized mice
  • Evaluation of antibody therapies targeting Fc?RI
  • Studying human monocyte/macrophage biology in vivo in a murine context

If your study involves standard immunocompetent mice (without human CD64 expression), clone 10.1 is not suitable for targeting mouse CD64—other clones targeting the mouse antigen should be used. For humanized or knock-in mouse models, clone 10.1 is a well-established tool for functional studies involving Fc?RI/CD64.

The correct storage temperature for sterile packaged items such as Clone 10.1 is between 18°C and 23°C (64°F–73°F), with relative humidity maintained between 30% and 60%.

This range is established to ensure sterile items remain uncontaminated and maintain their shelf-life. Items should be stored in enclosed, clean, dry environments, protected from dust, moisture, and direct light. If the packaging is damaged, wet, or otherwise compromised, sterility can no longer be guaranteed and the item should be reprocessed.

Some sterile medical device guidelines set the maximum storage room temperature at 25°C, emphasizing that the environment must be dry, clean, and protected from unsterile objects. Keeping the temperature below this threshold ensures the packaging's integrity.

Key points for sterile storage:

• Temperature: 18°C to 23°C (do not exceed 25°C).
• Humidity: 30% to 60% RH.
• Environment: Enclosed, clean, dry, dust-free, away from direct sunlight and moisture.
• Packaging integrity: Use-by/expiration dates are valid only if storage conditions are met and packaging remains undamaged.

Always consult any manufacturer-specific instructions or local institutional guidelines, if available, for particular clones or products, as these may specify additional requirements.

Clone 10.1 is most commonly used as an antibody against human CD64 (Fc?RI), and in the literature, it is frequently utilized alongside other immune cell markers, especially for immunophenotyping via flow cytometry or immunohistochemistry. Researchers often combine 10.1 with antibodies or proteins that delineate leukocyte subsets, immune activation, or functional state.

Key antibodies and proteins commonly used with 10.1 include:

• CD14: Monocyte/macrophage marker—often paired with CD64 to define monocyte populations.
• CD16 (Fc?RIII): Used to distinguish classical, intermediate, and non-classical monocytes when stratifying by CD14/CD16/CD64 profiles.
• HLA-DR: MHC class II molecule, important for identifying antigen-presenting cell subsets with or without CD64.
• CD45: Pan-leukocyte marker, frequently used as a backbone in flow cytometry panels.
• CD32 (Fc?RII): Other Fc receptor in the family, used to distinguish between Fc?R subclasses on leukocytes.
• Isotype controls: Matched mouse IgG1 ? or other isotypes for appropriate antibody specificity controls.

These combinations enable specific identification of monocyte subsets, activated macrophages, and dendritic cells in complex samples. Most studies cited with 10.1 usage in prominent references (such as Leucocyte Typing series, and peer-reviewed immunology literature) employ these panels to clarify immune cell phenotyping, investigate Fc receptor function, or examine immune responses in infection and inflammation.

Other commonly used marker/antibody panels can include:

• CD11b (integrin): Macrophage and granulocyte marker.
• CD33: Myeloid lineage marker.
• Secondary antibody conjugates: Such as anti-mouse IgG, when indirect detection is employed.

Selection of additional antibodies generally depends on the specific experimental goals (identification, functional analysis, imaging), and may also include conjugated variants (e.g., PE, FITC), depending on the detection method.

In summary, the core set of antibodies/proteins frequently used with 10.1 are CD14, CD16, HLA-DR, CD45, CD32, and cell type lineage markers, facilitating accurate immune cell characterization and functional studies.

The key scientific findings from literature citations of clone 10.1 relate primarily to its use as a monoclonal antibody specific for human CD64 (Fc?RI), which is widely utilized in immunology and cell biology research.

Essential findings from clone 10.1 citations:

• Epitope and Target: Clone 10.1 specifically recognizes the EC3 epitope of the CD64 molecule (Fc?RI), a high-affinity IgG receptor expressed predominantly on monocytes and macrophages.
• Binding Specificity: In-house testing suggests that clone 10.1 preferentially binds to the CD64A isoform of Fc?RI.
• Applications: The antibody is primarily cited for applications such as flow cytometry, immunohistochemistry, and functional assays aimed at identifying or isolating CD64-positive cells in human samples.
• Unique Reagent Identifier: It is indexed under RRID:AB_2536514 and is commercially available under various catalog numbers (e.g., Thermo Fisher CD6405).

Supporting context:

• Multiple literature references cite clone 10.1 for its performance and specificity in detecting human CD64, and these studies leverage it to characterize immune cell populations or study inflammatory responses.
• There are no major controversies or negative findings associated with clone 10.1 in the core scientific literature indexed in these sources; its citations are almost always for standard immunophenotyping or investigative research on monocyte/macrophage function.

Additional relevant information:

• Some commercial sources recommend specific papers citing clone 10.1 as technical references.
• The literature generally treats clone 10.1 as a validated, reliable reagent for CD64 analysis, with no reported cross-reactivity issues under standard conditions.

In summary, clone 10.1 is a well-characterized anti-CD64 monoclonal antibody with high specificity for the EC3 epitope, and its primary scientific impact is its utility in immunological studies involving monocytes and macrophages.