Antibody DetailsProduct DetailsReactivity Species Human Host Species Mouse Product Concentration ≥ 2.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829867 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this T3-3A1 antibody for staining cells in flow cytometry is ≤ 0.5 - 1 μg per 106 cells in a volume of 0.1 ml or 0.1 ml of whole blood. Titration of the reagent is recommended for optimal performance for each application. Other Applications Reported In Literature ? IF Staining The suggested concentration for this T3-3A1 antibody for use in Immunofluorescence Staining is ≤ 0.5 - 1.0 ug/ml Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone T3-3A1 recognizes an epitope on human CD7. Antigen Distribution CD7 is expressed on T cells, NK cells, thymocytes, hematopoietic progenitors, some normal B cells, malignant B cells, acute lymphocytic leukemia (ALL) cells, and some acute myeloid leukemia (AML) cells. It is weakly expressed on monocytes. Background CD7 is a 40 kD type I transmembrane glycoprotein and is a member of the Ig superfamily. It plays a vital role in T-cell interactions, in adition to, T-cell/B-cell interaction during early lymphoid development, and has been shown to interact with PIK3R1. Functional studies indicate that crosslinking of the CD7 can stimulate transmembrane calcium flux in T cells. Lack of CD7 expression can help to diagnose T-cell lymphoproliferative disorders, such as mycosis fungoides/Sezary syndrome and adult T-cell leukemia/lymphoma. Antigen DetailsProtein Ligand/Receptor Cytoplasmic domain associates with P13-kinase PubMed NCBI Gene Bank ID UniProt.org Research Area Costimulatory Molecules . Immunology Technical Protocols |
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