Antibody Details
Product Details
ImmunogenRecombinant human Cytokeratin 18.
FormulationTris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
Product PreparationPurified by immunoaffinity chromatography
Storage and HandlingStore at 2-8°C. Do not freeze.
Applications and Recommended Usage?
Quality Tested by Leinco
Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Description
SpecificityMouse Monoclonal Antibody specific to Cytokeratin 18
BackgroundCytokeratin 18 is a type I cytokeratin. Together with its filament partner cytokeratin 8, it is perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues. Mutations in this gene have been linked to cryptogenic cirrhosis. Multimers of cytokeratin 18 and cytokeratin 8 are present in simple, glandular, and transitional epithelial cells, but absent from stratified epithelial cells, and are found in adenocarcinomas of simple and glandular epithelium origin and poorly differentiated squamous carcinoma cells.
FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. {PubMed:15529338, PubMed:16424149, PubMed:17213200, PubMed:7523419, PubMed:8522591, PubMed:9298992, PubMed:9524113}.
References & Citations
Immunohistochemistry: Detection of Cytokeratin 18 in human breast carcinoma with #34058 diluted 1:100-1:200.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
