Anti-Human DFF40 (I18)

Anti-Human DFF40 (I18)

Product No.: D240

[product_table name="All Top" skus="D240"]

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Target
DFF40
Product Type
Polyclonal Antibody
Alternate Names
CAD, Caspase Activated Deoxyribonuclease
Applications
IHC FFPE
,
WB

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Select Product Size

Data

D240 Western Blot
D240 IHC Jurkat Cells
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rabbit
Immunogen
PN:D270
Product Concentration
0.5 - 1.0 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human DFF40 recognizes an epitope near the center of mouse, rat and human DFF40. This polyclonal antibody was purified using affinity chromatography.
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively.3,5 DFF45/ICAD is the inhibitory protein of DFF40/CAD1,2 and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

Antigen Details

PubMed

References & Citations

1. Enari, M. et al. (1998) Nature391:43-50
2. Sakahira, H. et al. (1998) Nature 391:96-99
3. Halenbeck, R. et al. (1998) Curr Biol 8:537-40
4. Liu, X. et al. (1998) Proc Natl Acad Sci USA 95:8461-6
IHC FFPE
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.