Anti-Human DFF45 (NT)

Anti-Human DFF45 (NT)

Product No.: D244

[product_table name="All Top" skus="D244"]

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Target
DFF45
Product Type
Polyclonal Antibody
Alternate Names
ICAD, DNA Fragmentation Factor
Applications
WB

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Data

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rabbit
Immunogen
PN:D272
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human DFF45 recognizes an epitope near the N-terminus of Human DFF45. This polyclonal antibody was purified using affinity chromatography.
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.

Antigen Details

PubMed

References & Citations

1. Liu, X. et al. (1997) Cell 89:175-184
2. Enari, M. et al. (1998) Nature 391:43-50
3. Sakahira, H. et al. (1998) Nature 391:96-99
4. Gu, J. et al. (1999) J Biol Chem 274:20759-62
General Western Blot Protocol
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Prod No.
Description
D243
D244
Products are for research use only. Not for use in diagnostic or therapeutic procedures.