Anti-Human Dtk (Clone 96201)

Product No.: D354


Clone 96201 Target Dtk Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Developmental Receptor Tyrosine Kinase Isotype IgG1 Applications ELISA Cap , FC , IHC FFPE


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human Dtk (Accession # Q06418) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829934 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:D366 at a concentration of approximately 300 µg/ml. A suggested standard for this assay is PN:D338. Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Developmental Receptor Tyrosine Kinase (Dtk) (Clone 96201) recognizes an epitope on Human Dtk. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Dtk belongs to a recently recognized subfamily of receptor tyrosine kinases that also includes Ufo (Axl/Ark) and Mer (Eyk). Ligands for Dtk and Ufo have been identified as protein S and the related molecule Gas6, respectively. Dtk is likely to be involved in the regulation of hematopoiesis, particularly during the embryonic stages of blood cell development. ¹ DTK is also abundantly expressed in adult kidney, testis, and ovary. ² PubMed Dtk NCBI Gene Bank ID 7301 UniProt.org Information on Uniprot.org References & Citations 1. Crosier, KE.et al.(1996)Exp Hematol24:318 2. Crosier, PS.et al.(1994)Growth Factors11:137 View product citations for antibody D354 on CiteAb Technical Protocols IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human Dtk (Accession # Q06418)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2829934

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:D366 at a concentration of approximately 300 µg/ml. A suggested standard for this assay is PN:D338.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Developmental Receptor Tyrosine Kinase (Dtk) (Clone 96201) recognizes an epitope on Human Dtk. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Dtk belongs to a recently recognized subfamily of receptor tyrosine kinases that also includes Ufo (Axl/Ark) and Mer (Eyk). Ligands for Dtk and Ufo have been identified as protein S and the related molecule Gas6, respectively. Dtk is likely to be involved in the regulation of hematopoiesis, particularly during the embryonic stages of blood cell development. ¹ DTK is also abundantly expressed in adult kidney, testis, and ovary. ²

PubMed

Dtk

NCBI Gene Bank ID

7301

UniProt.org

Information on Uniprot.org