Anti-Human E-Cadherin Antibody (34060)

Anti-Human E-Cadherin Antibody (34060)

Product No.: 34060

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Clone
G564.1
Target
E-Cadherin
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD antigen CD324 [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
Isotype
Mouse IgG1
Applications
IHC

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Select Product Size

Data

Immunohistochemistry: Detection of E-cadherin in human breast with #34060 diluted 1:100-1:200.
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Recombinant human E-cadherin.
Formulation
Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
Store at 2-8°C. Do not freeze.
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Monoclonal Antibody specific to E-cadherin
Background
E-cadherin is a calcium-dependent intercellular adhesion molecule encoded by the CDH1 gene and present on epithelial cells. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer. When compared with invasive ductal carcinoma, E-cadherin expression is markedly reduced or absent in the great majority of invasive lobular carcinomas when studied by immunohistochemistry.
Function
Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. {PubMed:11976333, PubMed:16417575}.; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. {PubMed:16417575}.; (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. {PubMed:10406800, PubMed:17540170, PubMed:8601315}.
NCBI Gene Bank ID
UniProt.org
Research Area
Cancer Research

References & Citations

Formats Available

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Prod No.
Description
34060
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.