Anti-Human EGFR (Clone EGFR.1) – Purified in vivo PLATINUM™ Functional Grade
Anti-Human EGFR (Clone EGFR.1) – Purified in vivo PLATINUM™ Functional Grade
Product No.: E337
Clone EGFR.1 Target EGFR Formats AvailableView All Product Type Monoclonal Antibody Alternate Names ERBB, ERBB1, mENA Isotype Mouse IgG2b κ Applications IHC , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen A431 cultured cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830095 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone EGFR.1 recognizes the human EGFR. Background EGFR is a 170 kD transmembrane glycoprotein that is part of the ErbB family of receptors within the protein kinase superfamily. EGFR is one of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR is essential for various processes including controlling cell growth and differentiation and ductal development of the mammary glands. Ligand binding induces dimerization and autophosphorylation. It consists of a glycosylated extracellular domain which binds to EGF and an intracellular domain with tyrosine-kinase activity necessary for signal transduction. TGFα, vaccinia virus growth factor, and related growth factors can also bind to and signal through EGFR. Abnormal EGFR signaling has been implicated in inflammatory diseases such as psoriasis, eczema and atherosclerosis. Alzheimer's disease is linked with poor signaling of the EGFR and other receptor tyrosine kinases. Furthermore, over-expression of the EGFR is linked with the growth of various tumors. EGFR has been identified as an oncogene, a gene which in certain circumstances can transform a cell into a tumor cell, which has led to the therapeutic development of anticancer EGFR inhibitors. EGFR is a well-established target for both mAbs and specific tyrosine kinase inhibitors. Ligand/Receptor Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Signal Transduction Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Use of Clone EGFR.1 in In Vivo Mouse StudiesThere is no direct evidence in the provided search results for a clone named "EGFR.1" being used in in vivo mouse studies. However, the results discuss related topics and antibodies that may clarify the scientific context. Evidence from Search Results
Typical Use of Anti-EGFR Clones in MiceWhile "EGFR.1" is not specifically documented here, in general, anti-EGFR monoclonal antibodies (clones) are used in mouse studies to:
Key Considerations
ConclusionThere is no evidence in the provided results that a clone named "EGFR.1" has been used in in vivo mouse studies. For such studies, researchers typically use either species-specific anti-EGFR antibodies (with demonstrated activity in mice), genetically engineered mouse models, or human xenografts in immunodeficient mice. Any new clone intended for in vivo use would need extensive preclinical validation to ensure it effectively targets mouse EGFR and elicits the desired biological effects. If you have a specific reference or context for "EGFR.1," more targeted information may be required. The correct storage temperature for the sterile packaged clone EGFR.1 depends on its formulation and labeling by the manufacturer, but for the commonly available anti-EGFR monoclonal antibody (Clone: EGFR.1), the recommended storage temperature is 2–8°C (refrigerated), protected from light, and it should not be frozen. Some additional context:
Always defer to the suppliers product datasheet for your specific batch to ensure correct storage. Most commercial EGFR.1 antibody datasheets (including conjugated forms) recommend storage at 2–8°C and specify not to freeze. Commonly used antibodies or proteins studied alongside EGFR (Epidermal Growth Factor Receptor)including when "EGFR.1" is referenced as a clone or reagentoften include other EGFR family members, EGFR-targeted drugs, and signaling pathway markers. Key proteins and antibodies frequently used in combination with EGFR antibodies in the literature include:
In cancer studies, EGFR is often used with markers for:
Summary table of commonly used antibodies/proteins with EGFR:
Note:
For highly specific companion antibodies with clone "EGFR.1" (if referencing a distinct antibody clone), literature sometimes pairs it directly with other anti-EGFR mAbs (e.g., clone 225/cetuximab) to probe for non-overlapping epitopes or to maximize receptor downregulation. Key findings from scientific literature citing clone EGFR.1 (a monoclonal antibody used against the Epidermal Growth Factor Receptor, EGFR) are centered on its applications in research and diagnostics, particularly in oncology. The literature on clone EGFR.1 itself is somewhat limited, but citations frequently discuss the following themes:
In summary, clone EGFR.1 is an important reagent for identifying EGFR expression in human tumors, contributing to diagnostics, prognostication, and research into targeted therapies. While molecular methods increasingly supplement or supplant protein-based detection for certain clinical decisions, antibodies like EGFR.1 underpin much of the foundational work and ongoing routine analysis in cancer pathology. References & Citations1. Berger, SM. et al. (1987) J. of Pathology 152:297 2. Downward, J. et al. (1984) Nature 311:483 3. Gullick, WJ. et al. (1985) EMBO J. 4:2869 4. Gullick, WJ. et al. (1986) Cancer Research 46:285 5. Gullick, WJ. et al. (1991) Br. Med. Bulletin 47:87 Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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E333 | |
E325 | |
E326 | |
E327 | |
E329 | |
E331 | |
E336 | |
E101 | |
E337 | |
E100 |
