Anti-Human EGFR – Purified in vivo PLATINUM™ Functional Grade

Anti-Human EGFR – Purified in vivo PLATINUM™ Functional Grade

Product No.: E337

[product_table name="All Top" skus="E337"]

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Clone
EGFR.1
Target
EGFR
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
ERBB, ERBB1, mENA
Isotype
IgG2b κ
Applications
in vivo
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
A431 cultured cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone EGFR.1 recognizes the human EGFR.
Background
EGFR is a 170 kD transmembrane glycoprotein that is part of the ErbB family of receptors within the protein kinase superfamily. EGFR is one of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR is essential for various processes including controlling cell growth and differentiation and ductal development of the mammary glands. Ligand binding induces dimerization and autophosphorylation. It consists of a glycosylated extracellular domain which binds to EGF and an intracellular domain with tyrosine-kinase activity necessary for signal transduction. TGFα, vaccinia virus growth factor, and related growth factors can also bind to and signal through EGFR. Abnormal EGFR signaling has been implicated in inflammatory diseases such as psoriasis, eczema and atherosclerosis. Alzheimer's disease is linked with poor signaling of the EGFR and other receptor tyrosine kinases. Furthermore, over-expression of the EGFR is linked with the growth of various tumors. EGFR has been identified as an oncogene, a gene which in certain circumstances can transform a cell into a tumor cell, which has led to the therapeutic development of anticancer EGFR inhibitors. EGFR is a well-established target for both mAbs and specific tyrosine kinase inhibitors.

Antigen Details

Ligand/Receptor
Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
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Signal Transduction

References & Citations

1. Berger, SM. et al. (1987) J. of Pathology 152:297
2. Downward, J. et al. (1984) Nature 311:483
3. Gullick, WJ. et al. (1985) EMBO J. 4:2869
4. Gullick, WJ. et al. (1986) Cancer Research 46:285
5. Gullick, WJ. et al. (1991) Br. Med. Bulletin 47:87
in vivo Protocol
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.