Anti-Human Epidermal Growth Factor Receptor (EGFR) (Clone F4) – Purified

Home/Primary Monoclonal Antibodies/Growth Factors and Cytokines/Anti-Human Epidermal Growth Factor Receptor (EGFR) (Clone F4) – Purified

Pricing & Details

Product No.E102
Clone
F4
Protein
EGFR
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
ErbB-1, HER1
Isotype
Mouse
IgG1
Applications
IHC FFPE
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
E102-0.25 mg
0.25 mg
$267.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
A peptide (residues 985 to 996 of human EGFR) coupled to keyhole limpet hemocyanin.
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-EGFR (clone F4) recognizes the internal domain with an epitope between amino acids 985-996 from the complete EGF receptor sequence of 1206 amino acids. It is thus about 200 residues from the C-terminus but just outside the region of sequence homology shared by the src oncogene family.4
Background
EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer - although there is some evidence that preformed inactive dimers may also exist before ligand binding. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. There is also evidence to suggest that clusters of activated EGFRs form, although it remains unclear whether this clustering is important for activation itself or occurs subsequent to activation of individual dimers. Over expression of the EGF-receptor is a hallmark of squamous cell carcinomas.

Antigen Details

Protein
PubMed
Research Area
Cancer
.
Cell Biology
.
Immunology
.
Innate Immunity
.
Neuroinflammation
.
Neuroscience

References & Citations

1. Berger, S. M. et al. (1987) J. of Pathology 152:297 2. Downward, J. et al. (1984) Nature 311:483 3. Gullick, W. J. et al. (1985) EMBO J. 4:2869 4. Gullick, W. J. et al. (1986) Cancer Research 46:285 5. Gullick, W. J. et al. (1991) Br. Med. Bulletin 47:87
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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