Anti-Human Epidermal Growth Factor Receptor (EGFR) – Biotin

Anti-Human Epidermal Growth Factor Receptor (EGFR) – Biotin

Product No.: E103

[product_table name="All Top" skus="E103"]

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Clone
F4
Target
Epidermal Growth Factor Receptor Internal Domain
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
ErbB-1, HER1
Isotype
IgG1
Applications
ELISA Det
,
IHC FFPE

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Select Product Size

Data

E102-E103 IHC
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-EGFR (clone F4) recognizes the internal domain with an epitope between amino acids 985-996 from the complete EGF receptor sequence of 1206 amino acids. It is thus about 200 residues from the C-terminus but just outside the region of sequence homology shared by the src oncogene family.4
Background
EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer - although there is some evidence that preformed inactive dimers may also exist before ligand binding. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. There is also evidence to suggest that clusters of activated EGFRs form, although it remains unclear whether this clustering is important for activation itself or occurs subsequent to activation of individual dimers. Over expression of the EGF-receptor is a hallmark of squamous cell carcinomas.

Antigen Details

Research Area
Cell Biology
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Immunology
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Innate Immunity
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Neuroinflammation
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Neuroscience

References & Citations

1. Berger, S. M. et al. (1987) J. of Pathology 152:297 2. Downward, J. et al. (1984) Nature 311:483 3. Gullick, W. J. et al. (1985) EMBO J. 4:2869 4. Gullick, W. J. et al. (1986) Cancer Research 46:285 5. Gullick, W. J. et al. (1991) Br. Med. Bulletin 47:87
ELISA Det
IHC FFPE

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.