Anti-Human ErbB3-Biotin

Anti-Human ErbB3-Biotin

Product No.: E275

[product_table name="All Top" skus="E275"]

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Target
ErbB3
Product Type
Polyclonal Antibody
Alternate Names
V-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian), ERBB3, HER3, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3
Applications
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human ErbB3 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human ErbB3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E197.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human ErbB3 recognizes Human ErbB3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), also known as ERBB3 is a member of the epidermal growth factor (EGF) receptor subfamily of receptor tyrosine kinases and is believed to be a receptor for an unknown ligand.1 ErbB3 has a heregulin (HRG) or neuregulin binding domain but lacks intrinsic protein tyrosine kinase activity.2 Therefore, it can bind to the ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity.
Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of ErbB3 and/or over-expression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors.

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Cerione, RA. et al. (1994) J Biol Chem. 269: 14303
2. Koland, JG. et al. (1998) Biochem J. 333: 757
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.