Pricing & Details
Purified Recombinant Human ErbB3 (>98%)
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing ErbB3. A suggested method would be to stain cells expressing ErbB3 with 2.5 µg/106 cells in a total staining volume of ≤200 µl followed by PN:G641.
Western Blotting: To detect Human ErbB3 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human ErbB3 is 1 ng/lane under non-reducing and 10 ng/lane under reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E197.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human ErbB3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human ErbB3 (at 10 ng/ml) is 0.125-0.5 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human ErbB3 recognizes Human ErbB3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), also known as ERBB3 is a member of the epidermal growth factor (EGF) receptor subfamily of receptor tyrosine kinases and is believed to be a receptor for an unknown ligand.1 ErbB3 has a heregulin (HRG) or neuregulin binding domain but lacks intrinsic protein tyrosine kinase activity.2 Therefore, it can bind to the ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of ErbB3 and/or over-expression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors.
NCBI Gene Bank ID
References & Citations
1. Cerione, RA. et al. (1994) J Biol Chem. 269: 14303
2. Koland, JG. et al. (1998) Biochem J. 333: 757
Products are for research use only. Not for use in diagnostic or therapeutic procedures.