Anti-Human FGF-22 (Clone 435008)

Product No.: F1132


Clone 435008 Target FGF-22 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Fibroblast Growth Factor-22 Isotype IgG2a Applications N , WB


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Data


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human FGF-22 (Accession # Q9HCT0) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830166 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human FGF-22 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF-22 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1110. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human FGF-22 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human FGF-22 (at 1 ng/ml) is 1.5-7.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human Fibroblast Growth Factor-22 (FGF-22) (Clone 435008) recognizes an epitope on Human FGF-22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed FGF-22 NCBI Gene Bank ID 27006 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody F1132 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human FGF-22 (Accession # Q9HCT0)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830166

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human FGF-22 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF-22 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1110.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human FGF-22 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human FGF-22 (at 1 ng/ml) is 1.5-7.5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Fibroblast Growth Factor-22 (FGF-22) (Clone 435008) recognizes an epitope on Human FGF-22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

FGF-22

NCBI Gene Bank ID

27006

UniProt.org

Information on Uniprot.org

References & Citations

View product citations for antibody F1132 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
F1119	Anti-Human FGF-22
F1132	Anti-Human FGF-22 (Clone 435008)
F1124	Anti-Human FGF-22 – Biotin

Formats Available

Prod No.	Description
F1132	Anti-Human FGF-22 (Clone 435008)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human FGF-22 (Clone 435008)