Anti-Human Fibulin-4 [Clone 5G11] — Purified in vivo PLATINUM™ Functional Grade

In in vivo mouse studies, the 5G11 clone is primarily used as a rat monoclonal antibody that specifically recognizes the mouse lymphotoxin beta receptor (LT?R), a member of the tumor necrosis factor receptor superfamily expressed mainly on stromal cells and fibroblasts. This antibody is reported to be agonistic, meaning it activates the LT?R pathway, leading to outcomes such as NF-?B activation, MIP-2 induction, and IL-6 release in experimental models.

Key uses and relevant context:

• Agonistic activity: The 5G11b (or 5G11) clone is used to experimentally activate LT?R signaling in mice, which can be relevant for studying immune modulation, lymphoid tissue organization, inflammation, or tumorigenesis.
• Functional readouts: In vivo administration of this antibody can be used to induce specific molecular responses (e.g., cytokine release) and examine the receptor’s role in mouse physiology and pathophysiology.
• Experimental controls: It is often tested on appropriate cell lines (like WEH164S) to validate its activity before in vivo use.

Note:

• There are other unrelated 5G11 antibody clones, such as an anti-human desmoglein 3 antibody, but these are not used in mouse in vivo studies and target a different protein/species.

In summary, clone 5G11 (specifically 5G11b) is used in vivo in mice to functionally activate the lymphotoxin beta receptor, enabling the study of LT?R-dependent signaling and biological effects.

The correct storage temperature for sterile packaged clone 5G11 monoclonal antibodies is aliquot and store at -20°C or -80°C, and avoid repeated freeze-thaw cycles.

For longer-term storage (months to years), -80°C is preferred to maximize stability. For short-term storage (up to several weeks), some sources mention that storage between 2°C and 8°C (standard refrigerator conditions) is possible, but this is less ideal for maintaining antibody activity over time.

Additional details:

• Store aliquots in the back of the freezer to minimize temperature fluctuations.
• Avoid frost-free freezers, as temperature cycling can degrade antibodies.
• If thawing aliquots, do not re-freeze; use a new aliquot to prevent denaturation.
• For certain applications and conjugates (e.g., fluorescent labels), short-term storage at 4°C is sometimes recommended, but always consult product-specific instructions for variations.

For clone 5G11 specifically, both anti-TOPORS and anti-desmoglein 3 monoclonal antibodies with this clone designation follow the same storage guidance above.

Based on the available information, I can identify several proteins and antibodies that are commonly used alongside 5G11 antibodies in research, though the search results show that 5G11 refers to different clone designations for distinct antibodies targeting different proteins.

Desmoglein-3 Research Context

The 5G11 clone targeting desmoglein-3 appears in several research applications. In western blotting experiments, GAPDH (D16H11) XP® Rabbit mAb is commonly used as a loading control alongside the Desmoglein 3 (5G11) antibody. This pairing is standard practice in protein analysis to normalize for protein loading variations between samples.

Fibulin-4 Research Applications

The 5G11 clone targeting Fibulin-4 is used across multiple immunological techniques including immunofluorescence, immunohistochemistry, and western blotting. However, the search results don't provide specific information about other antibodies commonly paired with this particular 5G11 clone in research protocols.

Research Method Considerations

The search results indicate that 5G11 antibodies are frequently used in combination with various technical controls and complementary reagents. For paraffin-embedded tissue sections, protein digestion treatments such as pepsin or pronase are commonly employed as pre-treatments, followed by heat-induced epitope retrieval (HIER) in citrate buffer when working with the desmoglein-3 targeting 5G11.

Unfortunately, the available search results don't provide comprehensive information about the broader antibody panels or protein interaction studies where 5G11 antibodies are routinely used. The literature would typically include information about co-staining antibodies, control antibodies, and related protein markers, but this specific information isn't detailed in the current search results for either the Fibulin-4 or Desmoglein-3 targeting versions of the 5G11 clone.

Clone 5G11 is a monoclonal mouse antibody that targets desmoglein 3 (DSG3), a protein involved in maintaining the integrity of epithelial tissues. Here are the key findings related to clone 5G11 from scientific literature:

1. Specificity and Use: Clone 5G11 is used as a primary antibody for staining and studying DSG3 in tissues. It specifically targets the terminal EC portion of DSG3, spanning amino acids 446-613.

2. Epitope Recognition: Clone 5G11 does not recognize the same epitope as other antibodies like 5H10, which targets a different region of DSG3 (amino acids 49-60).

3. Immunofluorescence and Immunohistochemistry: Clone 5G11 is used in techniques such as immunofluorescence and indirect immunoperoxidase staining to detect DSG3 in tissues, often in the context of studying pemphigus vulgaris, a disease characterized by autoantibodies against desmogleins.

4. Non-Cross-Reactivity with Other Antibodies: Clone 5G11 does not cross-react with other epitopes recognized by different antibodies, such as those identified in the competition ELISA experiments aimed at identifying distinct binding regions on DSG3.