Anti-Human Fibulin-4 – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.F2205
Clone
5G11
Protein
Fibulin-4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
EFEMP2; ARCL1B; FBLN4; FIBL-4; fibulin 4; MBP1; UPH1; EGF containing fibulin like extracellular matrix protein 2
Applications
IF Microscopy
,
IF Staining
,
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
F2205- 1mg
1.0 mg
$225.00
$175.50
In stock
Max:
Min: 1
Step: 1
F2205- 5mg
5.0 mg
$445.00
$347.10
In stock
Max:
Min: 1
Step: 1
F2205- 25mg
25 mg
$1,115.00
$869.70
In stock
Max:
Min: 1
Step: 1
F2205- 50mg
50 mg
$1,680.00
$1,310.40
In stock
Max:
Min: 1
Step: 1
F2205- 100mg
100 mg
$2,800.00
$2,184.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Recombinant, human Fibulin-4 produced in E. coli BL21 cells.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
WB The suggested concentration for this Fibulin-4 (Clone 5G11) antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
IF Staining
IF Microscopy
IHC FFPE
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 5G11 recognizes Human Fibulin-4. Monoclonal antibodies raised against the purified proteins did not show any crossreactivity with other family members.
Antigen Distribution
Fibulin-4 is predominantly expressed in tissues rich in elastic fibers.
Background
There are currently seven genes in the fibulin family (Fibulin-1, 2, 3, 4, 5, 6, and 7) that are associated with elastic fibers. The fibulins are widely distributed throughout the body and are more prominent in tissues rich in elastic fibers and are often associated with vasculature and elastic tissues. Fibulin-4 appears to be the most critical member of this family of proteins in modulating elastic-fiber formation because studies have shown that fibulin-4-null mice do not produce elastic fibers at all.1 This extracellular glycoprotein has a molecular weight of approximately 49 kD, and is not only considered a structural protein of the extracellular matrix, but also a regulatory factor required for normal elastin expression in human fibroblasts.2 Due to the regulatory role of Fibulin-4, it is thought that a pathophysiological link may exist between the modulation of fibulin-4 levels and diseases, such as WBS (Williams-Beuren syndrome), that are characterized by impaired elastic-fiber formation.1 Furthermore, fibulin-4 has been shown to interact with P53, a crucial homologous protein that prevents cancer formation, and thus functions as a tumor suppressor.3 One study indicated that colon tumors were found to have approximately 2-7-fold increases of fibulin-4 mRNA expression. This suggests that the dysregulated expression of the fibulin-4 gene is associated with human colon tumourigenesis.4

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Stem Cell

References & Citations

1. DiCorleto, PE. et al. (2009) Biochem J. 423(1): 79–89.
2. Kobayashi, N. et al. (2007) J Biol Chem. 282(16):11805-16.
3. Gallagher, WM. et al. (1999) Oncogene. 18(24):3608-16.
4. Gallagher, WM. et al. (2001) FEBS Lett. 489(1):59-66.
IHC
General Western Blot Protocol

Formats Available

Products are for research use only. N