Anti-Human Fibulin-4 – Purified

Anti-Human Fibulin-4 – Purified

Product No.: F2210

[product_table name="All Top" skus="C163"]

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Clone
5G11
Target
Fibulin-4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
EFEMP2; ARCL1B; FBLN4; FIBL-4; fibulin 4; MBP1; UPH1; EGF containing fibulin like extracellular matrix protein 2
Applications
IF Microscopy
,
IF Staining
,
IHC FFPE
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Immunogen
Recombinant, human Fibulin-4 produced in E. coli BL21 cells.
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
WB The suggested concentration for this Fibulin-4 (Clone 5G11) antibody for use in western blotting is 1-10 μg/ml.
Additional Applications Reported In Literature ?
IF Staining
IF Microscopy
IHC FFPE
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 5G11 recognizes Human Fibulin-4. Monoclonal antibodies raised against the purified proteins did not show any crossreactivity with other family members.
Antigen Distribution
Fibulin-4 is predominantly expressed in tissues rich in elastic fibers.
Background
There are currently seven genes in the fibulin family (Fibulin-1, 2, 3, 4, 5, 6, and 7) that are associated with elastic fibers. The fibulins are widely distributed throughout the body and are more prominent in tissues rich in elastic fibers and are often associated with vasculature and elastic tissues. Fibulin-4 appears to be the most critical member of this family of proteins in modulating elastic-fiber formation because studies have shown that fibulin-4-null mice do not produce elastic fibers at all.1 This extracellular glycoprotein has a molecular weight of approximately 49 kD, and is not only considered a structural protein of the extracellular matrix, but also a regulatory factor required for normal elastin expression in human fibroblasts.2 Due to the regulatory role of Fibulin-4, it is thought that a pathophysiological link may exist between the modulation of fibulin-4 levels and diseases, such as WBS (Williams-Beuren syndrome), that are characterized by impaired elastic-fiber formation.1 Furthermore, fibulin-4 has been shown to interact with P53, a crucial homologous protein that prevents cancer formation, and thus functions as a tumor suppressor.3 One study indicated that colon tumors were found to have approximately 2-7-fold increases of fibulin-4 mRNA expression. This suggests that the dysregulated expression of the fibulin-4 gene is associated with human colon tumourigenesis.4

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Stem Cell

References & Citations

1. DiCorleto, PE. et al. (2009) Biochem J. 423(1): 79–89.
2. Kobayashi, N. et al. (2007) J Biol Chem. 282(16):11805-16.
3. Gallagher, WM. et al. (1999) Oncogene. 18(24):3608-16.
4. Gallagher, WM. et al. (2001) FEBS Lett. 489(1):59-66.
IF Microscopy
IF Staining
IHC FFPE
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.