Anti-Human FLASH (CT)

PN:F145

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human FLASH recognizes and epitope near the C-terminus of Human FLASH. This polyclonal antibody was purified using affinity chromatography.

A novel mammalian CED-4 homologous was recently identified and cloned in mouse¹ and human² and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8.¹ There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity.^1,2 FLASH is widely expressed.

1. Imai, Y. et al. (1999) Nature 398:777-85
2. Koonin, EV. et al. (1999) Nature 401:662-3
3. Medema JP. (1999) Nature. 398(6730):756-7.