Anti-Human FLIP

PN:F148

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human FLIP-γ/δ recognizes an epitope near the C-terminus Human FLIP-γ/δ. This polyclonal antibody was purified using affinity chromatography.

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively.^1,2 The human FLIPs were also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and usurpin.^3-4 FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPα, β, γ, and δ, respectively.

1. Tschopp, J. et al. (1997) Nature. 386(6624):517-21.
2. Tschopp, J. et al. (1997) Nature. 388(6638):190-5.
3. Goeddel, DV. et al. (1997) Immunity. 6(6):751-63.
4. Dixit, VM. et al. (1997) J Biol Chem. 272 (28):17255-7.