Anti-Human FLIP

Product No.: F142

[product_table name="All Top" skus="F142"]

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Target
FLIP
Product Type
Polyclonal Antibody
Alternate Names
I-FLICE, FLAME-1, CASH, CLARP
Applications
IHC FFPE
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rabbit
Immunogen
PN:F148
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human FLIP-γ/δ recognizes an epitope near the C-terminus Human FLIP-γ/δ. This polyclonal antibody was purified using affinity chromatography.
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively.1,2 The human FLIPs were also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and usurpin.3-4 FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPα, β, γ, and δ, respectively.

Antigen Details

PubMed

References & Citations

1. Tschopp, J. et al. (1997) Nature. 386(6624):517-21.
2. Tschopp, J. et al. (1997) Nature. 388(6638):190-5.
3. Goeddel, DV. et al. (1997) Immunity. 6(6):751-63.
4. Dixit, VM. et al. (1997) J Biol Chem. 272 (28):17255-7.
IHC FFPE
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.