Anti-Human Hepsin (Clone 2D5) – Purified in vivo GOLD™ Functional Grade
Anti-Human Hepsin (Clone 2D5) – Purified in vivo GOLD™ Functional Grade
Product No.: H4400
Clone 2D5 Target Hepsin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names HPN, TMPRSS1, 2D5-1.9, 2D5.1.9 Isotype Mouse IgG2a k Applications ELISA , FC , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human hepsin protein Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830332 Applications and Recommended Usage? Quality Tested by Leinco ELISA FC WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 2D5 recognizes Human hepsin. It binds to a separate epitope than clone 3H10.1. These clones do not interfere with one another. Background Hepsin is a type II transmembrane serine protease (TTSP) expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues. The physiological function of hepsin is unclear, although, In vitro studies have shown that hepsin activates blood clotting factors VII, XII, and IX, pro-urokinase (pro-uPA), and pro-hepatocyte growth factor (pro-HGF). The over-expression of hepsin has been implicated in several types of cancer, especially ovarian and prostate, which makes it an attractive diagnostic marker for cancers. Most notably, hepsin has been identified as one of the most highly induced genes in prostate cancer, and this over-expression is correlated with the cancer progression and metastasis. Furthermore anti-hepsin antibodies have been shown to inhibit the invasion of human prostate cancer cells.1 Antigen Distribution Hepsin is expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues. PubMed NCBI Gene Bank ID UniProt.org Research Area Immuno-Oncology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 2D5 refers to several monoclonal antibodies with different specificities and uses, but in the context of in vivo mouse studies, the most well-documented application is as a mouse anti-human CD4 antibody used for receptor blocking and HIV-1 challenge models in nonhuman primates. It has also been applied for in vivo studies targeting hepsin (HPN) in humanized mouse models. Key details on in vivo use:
Important Considerations Before In Vivo Use:
If you refer to a different antigen or model, please provide the full antigen name or application context, as there are multiple unrelated "2D5" clones (e.g., anti-RANTES, anti-hepsin, anti-cadherin, viral antigens). The most prevalent in vivo application documented is for receptor blockade (especially CD4 or hepsin) in humanized or engineered mouse models. The correct storage temperature for a sterile packaged clone like 2D5 would typically depend on its biological nature and packaging. However, for most biological clones, especially those used in research or medical applications, storage conditions are generally recommended as follows:
For sterile packaged medical devices or similar products, the storage conditions are more about maintaining sterility than temperature alone. They should be stored in a clean, dry environment, protected from light and dust, ideally at a room temperature not exceeding 25°C. Without specific details on the clone's packaging or nature, it is generally safest to follow the ultra-low freezer option for long-term preservation or consult the manufacturer's guidelines for precise instructions. In the literature, 2D5 is associated with different contexts, primarily involving monoclonal antibodies and peptides. Here are some commonly used antibodies or proteins mentioned alongside or in similar studies to 2D5:
These examples illustrate that 2D5 can be used in various contexts, from cancer research to viral immunology, often alongside other antibodies or proteins to enhance research outcomes. There is insufficient information in the provided search results to determine the key findings from clone 2D5 citations in scientific literature. None of the results directly reference "clone 2D5" or specific findings related to its use or citations in scientific research.
Given this, a focused search for "clone 2D5"–which is often an antibody clone used in immunology and molecular biology (e.g., for detecting certain cell markers)–would be needed to provide a summary of its scientific impact, applications, or key findings related to its use. If you are referring to a specific antibody (such as anti-mouse CD49d, clone 2D5, or an anti-human protein), please clarify or provide more context so that a targeted summary can be produced. Based on the current results, no key findings on clone 2D5 citations are available. For a comprehensive and accurate answer, more precise or directly relevant sources would be required. References & Citations1. Wu, Q. and Parry, G. (2007) Front Biosci 12 5052-9 Technical ProtocolsCertificate of Analysis |
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