Anti-Human Hepsin (Clone 2D5) – Purified in vivo GOLD™ Functional Grade

Anti-Human Hepsin (Clone 2D5) – Purified in vivo GOLD™ Functional Grade

Product No.: H4400

- -
- -
Clone
2D5
Target
Hepsin
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
HPN, TMPRSS1, 2D5-1.9, 2D5.1.9
Isotype
Mouse IgG2a k
Applications
ELISA
,
FC
,
WB

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
Human hepsin protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA
FC
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone 2D5 recognizes Human hepsin. It binds to a separate epitope than clone 3H10.1. These clones do not interfere with one another.
Background
Hepsin is a type II transmembrane serine protease (TTSP) expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues. The physiological function of hepsin is unclear, although, In vitro studies have shown that hepsin activates blood clotting factors VII, XII, and IX, pro-urokinase (pro-uPA), and pro-hepatocyte growth factor (pro-HGF). The over-expression of hepsin has been implicated in several types of cancer, especially ovarian and prostate, which makes it an attractive diagnostic marker for cancers. Most notably, hepsin has been identified as one of the most highly induced genes in prostate cancer, and this over-expression is correlated with the cancer progression and metastasis. Furthermore anti-hepsin antibodies have been shown to inhibit the invasion of human prostate cancer cells.1
Antigen Distribution
Hepsin is expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues.
PubMed
NCBI Gene Bank ID
Research Area
Immuno-Oncology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone 2D5 refers to several monoclonal antibodies with different specificities and uses, but in the context of in vivo mouse studies, the most well-documented application is as a mouse anti-human CD4 antibody used for receptor blocking and HIV-1 challenge models in nonhuman primates. It has also been applied for in vivo studies targeting hepsin (HPN) in humanized mouse models.

Key details on in vivo use:

  • CD4 Receptor Blocking in HIV Studies:
    The 2D5 clone has been used to block the CD4 receptor on T cells in macaques and potentially in mice engineered to express human CD4 for HIV-1 or SHIV challenge studies. In one study, 2D5 was administered systemically to achieve saturation of the CD4 receptor, aiming to prevent viral entry and thus infection after viral challenge. The antibody’s protective window was limited by its relatively short half-life and the need for very high tissue concentrations to achieve full receptor occupancy, especially in mucosal and peripheral lymphoid tissues.

  • Functional Grade Antibody for Hepsin (HPN):
    The anti-human hepsin clone 2D5 has been formulated specifically for in vivo use (i.e., devoid of preservatives like sodium azide and low in endotoxin), to ensure safety and functional activity when injected into mice. It is used to target human hepsin in tumor or tissue models, binding to a distinct epitope and not interfering with other anti-hepsin clones.

Important Considerations Before In Vivo Use:

  • Formulation: Antibody for in vivo studies must not contain sodium azide or significant endotoxin, as these can be toxic in animals.
  • Tissue Penetrance & Dosing: Achieving full occupancy/blockade of the target molecule in all relevant tissues may require high or repeated dosing.
  • Functional Purpose: Depending on the study, the antibody may be used for blocking, depletion, or neutralization, and protocols vary by target and model.

If you refer to a different antigen or model, please provide the full antigen name or application context, as there are multiple unrelated "2D5" clones (e.g., anti-RANTES, anti-hepsin, anti-cadherin, viral antigens). The most prevalent in vivo application documented is for receptor blockade (especially CD4 or hepsin) in humanized or engineered mouse models.

The correct storage temperature for a sterile packaged clone like 2D5 would typically depend on its biological nature and packaging. However, for most biological clones, especially those used in research or medical applications, storage conditions are generally recommended as follows:

  • Ultra-Low Freezer Storage: For long-term storage, an ultra-low freezer at approximately -80°C is often recommended. This temperature range can prevent degradation of biological molecules over extended periods.
  • Refrigerated Storage: For short-term storage, refrigeration at temperatures between 2°C and 8°C may be suitable, but this is typically not recommended for long-term storage without additional stabilizers.

For sterile packaged medical devices or similar products, the storage conditions are more about maintaining sterility than temperature alone. They should be stored in a clean, dry environment, protected from light and dust, ideally at a room temperature not exceeding 25°C.

Without specific details on the clone's packaging or nature, it is generally safest to follow the ultra-low freezer option for long-term preservation or consult the manufacturer's guidelines for precise instructions.

In the literature, 2D5 is associated with different contexts, primarily involving monoclonal antibodies and peptides. Here are some commonly used antibodies or proteins mentioned alongside or in similar studies to 2D5:

  1. PRAME Antibody (2D5 mAb):

    • This antibody is used for targeting PRAME (Preferentially Expressed Antigen in Melanoma) and is noted for its high affinity and specificity.
  2. 2D5 Peptide:

    • This peptide is mentioned as an anticancer agent that inhibits STAP-2–EGFR interactions. In studies, it is often used in conjunction with other antibodies in immunoblotting experiments, such as anti-Myc, anti-HA, anti-EGFR, and others.
  3. Polyclonal Antibodies:

    • In some studies, polyclonal antibodies like those from Abcam (e.g., ab89097) are compared to monoclonal antibodies like 2D5 for their binding capabilities.
  4. Vaccinia Virus Proteins:

    • While not directly related to 2D5, other studies involve antibodies against vaccinia virus proteins (A33, B5, L1), which are used to understand immune responses against viral infections.

These examples illustrate that 2D5 can be used in various contexts, from cancer research to viral immunology, often alongside other antibodies or proteins to enhance research outcomes.

There is insufficient information in the provided search results to determine the key findings from clone 2D5 citations in scientific literature. None of the results directly reference "clone 2D5" or specific findings related to its use or citations in scientific research.

  • The second result discusses clonal hematopoiesis (CH) and its impact on heart failure prognosis, detailing how even small clones identified by new sequencing methods have clinical significance. However, it does not mention clone 2D5.
  • The third result analyzes trends in replicability and citations in scientific literature, highlighting that nonreplicable papers are often cited more than replicable ones, but again does not reference clone 2D5 or related antibodies.

Given this, a focused search for "clone 2D5"–which is often an antibody clone used in immunology and molecular biology (e.g., for detecting certain cell markers)–would be needed to provide a summary of its scientific impact, applications, or key findings related to its use. If you are referring to a specific antibody (such as anti-mouse CD49d, clone 2D5, or an anti-human protein), please clarify or provide more context so that a targeted summary can be produced.

Based on the current results, no key findings on clone 2D5 citations are available. For a comprehensive and accurate answer, more precise or directly relevant sources would be required.

References & Citations

1. Wu, Q. and Parry, G. (2007) Front Biosci 12 5052-9
Indirect Elisa Protocol
Flow Cytometry
General Western Blot Protocol

Certificate of Analysis

Formats Available

- -
- -
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.