Anti-Human I-Kappa-B Kinase Epsilon (CT) (IKKε)

PN:I-327

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human I-Kappa-B Kinase Epsilon (IKKε) recognizes an epitope near the C-terminus of Human IKKε. This polyclonal antibody was purified using affinity chromatography.

Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-κB activity. IkB is phosphorylated by IkB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i.^1,2 IKKe is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1.¹ IKKε/IKK-i message is supexpressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.²

1. Peters, RT. et al. (2000) Mol. Cell 5(3):513
2. Shimada, T. et al. (1999) Int. Immunol. 11(8):1357