This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human I-Kappa-B Kinase Gamma (IKKγ) recognizes an epitope near the N-terminus of Human, Mouse and Rat IKKγ. This polyclonal antibody was purified using affinity chromatography.
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-κB activity. The IkB kinase (IKKα and IKKβ) phosphorylates IkB and mediates NF-κB activation. A novel molecule in the IKK complex was recently identified and termed IKKγ/NEMO/FIP3/IKKAP1.1-4 IKKg interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation.1-4 FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation.3
References & Citations
1. Rothwarf, DM. et al. (1998) Nature 395(6699):297
2. Yamaoka, S. et al. (1998) Cell 93(7):1231
3. Li, Y. et al. (1999) Proc. Natl. Acad. Sci. USA 96(3):1042
4. Mercurio, F. et al. (1999) Mol. Cell Biol. 19(2):1526
Products are for research use only. Not for use in diagnostic or therapeutic procedures.