Anti-Human ICOS

Product No.: I-611

[product_table name="All Top" skus="I-611"]

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Target
ICOS
Product Type
Polyclonal Antibody
Alternate Names
Inducible Co-Stimulator, AILIM
Applications
IHC FFPE
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human ICOS (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human ICOS this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human ICOS is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-551.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Inducible Costimulator (ICOS) recognizes Human ICOS. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Inducible co-stimulator (ICOS) is an inducible homodimeric costimulator protein that is structurally and functionally related to CD28. It is a third member of the CD28/CTLA4 family and it is expressed on activated T cells.1
ICOS regulates CD28-dependent and CD28-independent CD4 (+) subset (Th1 and Th2) responses.2 ICOS is a major regulator of the adaptive immune system and therefore, has a protective role in inflammatory autoimmune diseases.3

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Flavell, RA. et al. (2001) Nature 409: 97
2. Bachmann, MF. et al. (2000) J Exp Med. 192: 53
3. Kroczek, RA. et al. (1999) Nature 397: 263
IHC FFPE
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.