Recombinant Mouse ICOS

Recombinant Mouse ICOS

Product No.: I-550

[product_table name="All Top" skus="I-550"]

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Alternate Names
Inducible Co-Stimulator, H4, AILIM, Ly115
Product Type
Recombinant Protein
Expression Host
sf Insect Cells
Species
Mouse

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Product Size
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Background

Inducible co-stimulator (ICOS), also called ALIM and H4, is a homodimeric protein and member of the CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. ICOS enhances all basic T cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. Unlike the constitutively expressed CD28, ICOS has to be de novo induced on the T cell surface, does not up-regulate the production of IL-2, but superinduces the synthesis of IL-10, a B cell-differentiation factor (1). In vivo, ICOS is highly expressed on tonsillar T cells, which are closely associated with B cells in the apical light zone of germinal centres, the site of terminal B cell maturation. ICOS regulates CD28-dependent and CD28-independent CD4 (+) subset (Th1 and Th2) responses (2). It is a major regulator of the adaptive immune system and therefore, has a protective role in inflammatory autoimmune diseases (3).

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
eingsadhrm fsfhnggvqi sckypetvqq lkmrlfrere vlceltktkg sgnavsiknp mlclyhlsnn svsfflnnpd ssqgsyyfcs lsifdpppfq ernlsggylh iyesqlccql kliegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgk
N-terminal Sequence Analysis
Glu21
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse ICOS is Mr 44 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 47 kDa.
Predicted Molecular Mass
44
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Flavell, RA. et al. (2001) Nature 409:97
2. Bachmann, MF. et al. (2000) J. Exp. Med. 192:53
3. Kroczek, RA. et al. (1999) Nature 397:263
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.