Anti-Human CD119 (IFN-γ Rα Chain) – Purified in vivo PLATINUM™ Functional Grade
Anti-Human CD119 (IFN-γ Rα Chain) – Purified in vivo PLATINUM™ Functional Grade
Product No.: G737
Clone GIR-208 Target IFNγ Rα Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IFNGR1, IFN-γR, IFN-γRα, IFN-α R1, CDw119 Isotype Mouse IgG1 Applications FA , FC , in vivo |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human IFN-γRα, Purified from human placenta Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830301 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for clone GIR-208 purified antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? FA The In vivo PLATINUM™ Purified antibody ) is recommended for functional assays. Clone GIR-208 can be used as an isotype with Clone MAR1-5A3 (Anti-Mouse IFNAR1) Additional Reported Applications For Relevant Conjugates ? WB IP IHC B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone GIR-208 recognizes an epitope on human IFNγ Rα. Background IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and ß chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription. Antigen Distribution The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes. NCBI Gene Bank ID UniProt.org Research Area Immunology . Other Molecules Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone GIR-208 is a mouse monoclonal antibody that targets the human interferon-gamma receptor alpha (IFNγ Rα). In in vivo mouse studies, GIR-208 is often used as an isotype control rather than a specific therapeutic or diagnostic agent. This is because it does not interact with mouse interferon-gamma receptors but can serve as a control to assess the specificity of other treatments or antibodies in mouse models. It is also used in functional assays, such as flow cytometry, where it helps in staining cells to assess various cell surface markers. Additionally, GIR-208 can be used in research settings where understanding the effects of blocking or modulating specific immune pathways is necessary, although it specifically targets human receptors and is used in contexts where such specificity is needed. Common Applications:
However, since GIR-208 targets human receptors, its direct application in mouse models is limited to serving as a control or in studies involving humanized mice or specific research contexts where human receptor interactions are relevant. Commonly Used Antibodies and Proteins with GIR-208 in the LiteratureGIR-208 is a monoclonal antibody targeting the human interferon-gamma receptor alpha chain (IFN-γRα, CD119). It is widely used for flow cytometry, Western blotting, and functional studies involving IFN-γ signaling. Companion Antibodies
Other Proteins in IFN-γ Signaling Pathways
Non-Interferon Proteins
Summary Table
Additional Notes
In summary, the most commonly cited companion reagents for GIR-208 are GIR-301, isotype controls like MOPC-21, recombinant IFN-γ, and antibodies targeting the IFN-γRβ chain, reflecting its central role in IFN-γ receptor biology studies. Clone GIR-208 is a monoclonal antibody widely used in scientific literature to target human IFNγ receptor 1 (CD119, IFNγ Rα) in immunological research, particularly for studying interferon gamma (IFNγ) signaling pathways and related functional assays. The key findings from its citations are summarized as follows:
In summary, scientific citations of clone GIR-208 consistently highlight its reliability and versatility in targeting human IFNγ receptor 1, enabling mechanistic studies of IFNγ signaling, disease susceptibility related to IFNγR1, and immune cell profiling in both basic and translational immunology. Dosing regimens for clone GIR-208 in mouse models are highly standardized; published studies and commercial antibody providers report the use of a single 2 mg intraperitoneal (i.p.) dose per mouse, most often administered one day prior to experimental intervention. There is no documented variation in dose, frequency, or route of administration across different mouse strains or disease models within the available literature. Supporting Details:
Additional Information:
Comparison to Other Antibodies:
References & Citations1. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed 2. Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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I-977 | |
I-442 | |
I-969 | |
I-970 | |
I-971 | |
I-973 | |
I-975 | |
I-441 | |
I-443 | |
G737 |
