Anti-Human IgA (Clone HP6123) – Purified No Carrier Protein

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

Clone HP6123 activity is directed against both immunoglobulin A1 (IgA1) and immunoglobulin A2 (IgA2). HP6123 reacts against secretory and serum IgA.

IgA antibodies protect epithelial surfaces from infection by preventing the attachment of bacteria, viruses, and other pathogens to epithelial cells as well as by neutralizing bacterial toxins at mucosal surfaces¹. IgA antibodies also provide protection against infection in newborns via the ingestion of breast milk, into which IgA is naturally secreted. IgA can also act as an opsonin and weak complement activator as part of the immune defense response.

IgA-secreting plasma cells are found in lamina propria, a type of connective tissue located immediately below the basement membrane of surface epithelia¹. Once secreted, IgA antibodies are transported to the external surface of the epithelium as a dimer, where they bind to the poly- Ig receptor present on the basolateral surface of epithelial cells. Transcytosis and cleavage of the receptor then occur, which releases the extracellular portion of the Ig receptor while still attached to the Fc region of IgA. This molecule can then diffuse into the extracellular space and drain into the bloodstream.

IgA is produced by B lymphocytes. IgA is secreted by connective tissue, especially intestinal and respiratory tracts as well as lactating breast tissue. IgA is the major Ig isotype present in extracellular fluids secreted by epithelia and is also present in serum.

Ig receptor, heavy and light chains

1 Janeway CA Jr, Travers P, Walport M, et al. Immunobiology: The Immune System in Health and Disease. 5th edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available from: https://www.ncbi.nlm.nih.gov/books/NBK27162/
2 Kleine-Tebbe J, Hamilton RG, Roebber M, et al. J Immunol Methods. 179(2):153-164. 1995.
3 McIlwain DR, Chen H, Rahil Z, et al. Cell Host Microbe. 29(12):1828-1837.e5. 2021.
4 Goh YS, Ng LFP, Renia L. STAR Protoc. 2(3):100671. 2021.