Anti-Human IL-17A (Ixekizumab) – Fc Muted™

Anti-Human IL-17A (Ixekizumab) – Fc Muted™

Product No.: I-1235

- -
- -
Product No.I-1235
Clone
LY2439821
Target
IL-17A
Product Type
Biosimilar Recombinant Human Monoclonal Antibody
Alternate Names
CTLA8, interleukin 17A
Isotype
Human IgG4κ
Applications
ELISA
,
FA
,
FC
,
IF
,
WB

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Human
Host Species
HEK 293
FC Effector Activity
Muted
Immunogen
Humanized antibody from mouse clone 2321
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% by SDS Page
≥95% monomer by analytical SEC
Formulation
This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Recombinant biosimilar antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s recombinant biosimilar antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2-8°C Wet Ice
Additional Applications Reported In Literature ?
ELISA,
FA,
IF,
FC,
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Ixekizumab.

Ixekizumab has similar binding affinity to and neutralization of human and cynomolgus monkey IL-17A as well as weak binding to rabbit IL-17A. No binding is detected against rat or mouse IL-17A.
Background
IL-17 is a group of proinflammatory cytokines (IL-17A to IL-17F) released by T helper 17 (Th17) cells1. IL-17A is the key effector cytokine of the group 1 and is involved in normal inflammatory and immune responses2. Additionally, increased IL-17A plays an important role in the pathogenesis of ankylosing spondylitis (AS), a chronic autoimmune inflammatory disease that primarily affects the axial skeleton2, and in the progression of psoriatic arthritis1 and plaque psoriasis3, 4.

Ixekizumab was developed as an IL-17A inhibitor for the treatment of AS, psoriasis, and psoriatic arthritis and has been approved for the treatment of some patients with plaque psoriasis3, psoriatic arthritis, AS, and non-radiographic axial spondyloarthritis4. Ixekizumab is a fully humanized monoclonal antibody that binds selectively to IL-17A and inhibits its interaction with the IL-17 receptor, thereby inhibiting the release of proinflammatory cytokines and chemokines2, 3. Ixekizumab disrupts the proinflammatory cascade present in psoriasis4, resulting in decreased expression of cytokines from multiple T cell subsets as well as decreased keratinocyte proliferation and differentiation5.

Antigen Distribution
IL-17A is expressed by Th17 cells, mast cells, and neutrophils.
Ligand/Receptor
Il-17R
NCBI Gene Bank ID
UniProt.org
Research Area
Autoimmune
.
Biosimilars
.
Cancer
.
Immunology
.
Inflammatory Disease

References & Citations

1 Aboobacker S, Kurn H, Al Aboud AM. Secukinumab. [Updated 2023 Jun 20]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK537091/
2 Blair HA. Drugs. 79(4):433-443. 2019.
3 Markham A. Drugs. 76(8):901-905. 2016.
4 Preuss CV, Quick J. Ixekizumab. [Updated 2023 Jul 4]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK431088/
5 Krueger JG, Fretzin S, Suárez-Fariñas M, et al. J Allergy Clin Immunol. 130(1):145-54.e9. 2012.
6 Genovese MC, Van den Bosch F, Roberson SA, et al. Arthritis Rheum. 62(4):929-939. 2010.
7 Wang CQF, Suárez-Fariñas M, Nograles KE, et al. J Invest Dermatol. 134(12):2990-2993. 214.
8 Beerli RR, Bauer M, Fritzer A, et al. MAbs. 6(6):1608-1620. 2014.
9 Clarke DO, Hilbish KG, Waters DG, et al. Reprod Toxicol. 58:160-173. 2015.
Indirect Elisa Protocol
FA
Flow Cytometry
IF
General Western Blot Protocol

Certificate of Analysis

Formats Available

- -
- -
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.