Anti-Human IL-18 (Clone CPTC-IL18-4) – Purified No Carrier Protein

IL-18 synthetic peptide ISTLS(Cam)ENK

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

SAIC-24B-4 H2/L2 activity is directed against human IL18 peptide sequence ISTLSCENK.

CPTC-IL18-4

IL18 is an inducible, inflammatory cytokine that acts in homeostasis, barrier maintenance, alarmin activity, as an enhancer of immune responses to pathogens, and as an amplifier of inflammatory responses¹. IL18 is a member of the IL1 family of cytokines and is abundantly found at the mucosa and sites of inflammation. IL18 is activated by inflammasomes and other proteases, but unlike most cytokines lacks a signal peptide and must travel across the cell membrane through pores to elicit signaling. IL18 production differs depending on the tissues and inflammatory signals received. Inflammatory signals such as lipopolysaccharides and interferons can induce IL18. High IL18 levels are observed in many infectious, malignant, autoimmune, and autoinflammatory diseases. IL18 blockade is being investigated as a treatment for patients with rheumatic and oncological diseases.

SAIC-24B-4 H2/L2 was generated in rabbit for use in immuno-MRM assays using a synthetic peptide derived from human IL18 that is carbamidomethylated at the cysteine, ISTLSC[cam]ENK². SAIC-24B-4 H2/L2 also detects IL18 by indirect ELISA, but not by Western blotting³.

IL18 is produced by many cell types of both hematopoietic and non- hematopoietic origin. IL18 is particularly abundant in the barrier epithelial cells of the skin, upper airway, and gastrointestinal tract. The IL18 precursor protein is cytosolic. Once activated, the mature form is secreted and released to the extracellular milieu.

IL18R1, IL18RAP

1 Landy E, Carol H, Ring A, et al. Nat Rev Rheumatol. 20(1):33-47. 2024.
2 Whiteaker JR, Lundeen RA, Zhao L, et al. Front Immunol. 12:765898. 2021.
3 https://antibodies.cancer.gov/detail/CPTC-IL18-4#CPTC-IL18-4